Staphylococcal Toxins in Atopic Dermatitis and Eczema Herpeticum

Poitiers University Hospital41 enrolled

Overview

Atopic dermatitis (AD) is the most common chronic inflammatory skin disease. Clinical studies have demonstrated a link between staphylococcal skin colonization and the pathogenesis of AD, but the implication of bacterial virulence factors remains largely uncharacterized. Finally, AD is often associated with herpes simplex skin infections. The aim of this project is to investigate the role of staphylococcal toxins in the exacerbation and maintenance of atopic skin inflammation and in the occurrence of infectious complications such as eczema herpeticum.

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Atopic Dermatitis Staphylococcus Aureus Bacterial Toxins Immune Response Cytokines

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Patients with moderate AD (SCORAD between 25 and 50) or severe AD (SCORAD\> 50) * Skin lesions in the forearms * Free subject, without neither guardianship, wardship nor subordination * Patient with Social Security * Informed and signed consent by the patient after clear and loyal information on the study Exclusion Criteria: * Age \< 18 year-old * Patients with mild AD (SCORAD \< 25) * Patients without skin lesions in the forearms * Patients treated with dermocorticoid or calcineurin inhibitor for less than two weeks * Patients under systemic treatment : Methotrexate, Ciclosporin, Mycophenolate Mofetil, Azathioprine, general corticosteroids for less than 4 weeks * Patients under biological treatment : Dupilumab for less than 5 half-lives * Patient without Social Security * Pregnant and nursing women

Outcomes

Primary Outcomes

Quantification of S. aureus colonization level and characterization of bacterial virulence profile in AD lesions.

Biological data obtained from lesional skin will be compared with those of healthy skin.

Time frame: 2 hours (with consultation and sample)

Secondary Outcomes

Determination of the inflammatory profile of skin and blood during AD. Definition of the seric cytokine signature characteristic of AD. Characterization of the phenotype and function of the lymphocytic infiltrate T during AD.