Use of Microscopy, Cultures and Molecular Biological Methods for Diagnosing Nosocomial Pneumonia

Zealand University Hospital22 enrolled

Overview

Nosocomial pneumonia in the Intensive Care Unit is often not properly diagnosed mainly due to ongoing antimicrobial therapy. The study investigates the feasibility of more advanced diagnostic technics.

ICU patients fulfilling the CDC criteria for nosocomial pneumonia and mechanically ventilated for no more than 24 hours are included in the study. The material for the diagnostic procedure is pulmonal secretion collected by 1. suctioning in the tracheal tube and 2. bronchioalveolar lavage. The diagnostic procedures are culturing, 16S and18S gene PCR and subsequent sequencing. The 3 diagnostic methods will be compared in order of proper diagnosis and the ability to determine a specific antimicrobial treatment.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Nosocomial Pneumonia

Interventions

Microbiological diagnostics on pulmonary secretionDIAGNOSTIC_TEST

Culture, 16s, 18s, next generation sequencing

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Patients intubated because of respiratory failure following nosocomial pneumonia Exclusion Criteria: * Terminal patients * Patients with lung cancer * Intubated for more than 24 hours at the sampling time * Not fulfilling the CDC criteria for nosocomial pneumonia

Locations (1)

Zealand University Hospital

Køge, Region Sjælland, Denmark

Outcomes

Primary Outcomes

Ratio of patients where the advanced methods are superior to routine

Ratio of patients diagnosed by 16s, 18s or NGS to total number of patients compared to ratio of patients diagnosed by routine method (culture)

Time frame: 1 day

Data from ClinicalTrials.gov

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