First in Human Study of ChAdOx1-HBV in Healthy Participants and Participants With Chronic hepB Infection

Inclusion Criteria: 1. Adult males or females aged ≥18 to ≤65 years at screening 2. Body Mass Index ≤30 kg/m2 3. Able to provide informed consent indicating they understand the purpose of, and procedures required, for the study and are willing to participate 4. If female, willing not to become pregnant up to 8 weeks after last dose of study vaccine, not breast feeding 5. If female: Not pregnant, and one of the following: * Of non-childbearing potential (i.e. women who have had a hysterectomy or tubal ligation or are post menopausal, as defined by no menses in ≥1 year) * Sexual abstinence, only if the participant refrains from heterosexual intercourse during the entire study period and it is the usual lifestyle of the participant * Of childbearing potential but agrees to practice highly effective contraception for 4 weeks prior to study vaccine and 8 weeks after study vaccine. Highly effective methods of contraception include one or more of the following: Male partner who is sterile (medically effective vasectomy) prior to the female participant's entry into the study and is the sole sexual partner for the female participant, Hormonal (oral, intravaginal, transdermal, implantable or injectable), An intrauterine hormone releasing system, An intrauterine device and Bilateral tubal occlusion Healthy participants (cohorts 1 and 2): 6. Considered to be healthy with no current conditions that may significantly impair participant safety or influence study results, in the opinion of the Investigator Participants with well controlled CHB (cohorts 3 and 4): 7. Documented evidence of chronic HBV infection (e.g. HBsAg positive ≥6 months with detectable HBsAg levels at screening) 8. Receipt of only either entecavir or tenofovir for at least 12 months before screening 9. Virally suppressed (HBV DNA \<40 IU/mL for ≥6 months) 10. HBsAg \<4000IU/mL Participants with well controlled CHB (cohorts 3 and 4): 7\. Documented evidence of chronic HBV infection (e.g. HBsAg positive ≥6 months with detectable HBsAg levels at screening) 8. Receipt of only either entecavir or tenofovir for at least 12 months before screening 9. Virally suppressed (HBV DNA \<40 IU/mL for ≥6 months) 10. HBsAg \<10000 IU/mL Healthy participants (cohort 5): 11\. Considered to be healthy with no current conditions that may significantly impair participant safety or influence study results, in the opinion of the Investigator 12. Adult males or females aged ≥40 to ≤60 years at screening 13. Completed second dose of COVID-19 AZD1222 vaccine 10 to 18 weeks before enrolment Healthy participants (cohort 6): 14\. Considered to be healthy with no current conditions that may significantly impair participant safety or influence study results, in the opinion of the Investigator 15\. Adult males or females aged ≥40 to ≤60 years at screening 16\. Received the latest dose of Completed of either Pfizer (Comirnaty®) or Moderna (Spikevax) mRNA COVID 19 vaccine 6 to 30 weeks before enrolment Exclusion Criteria: 1. Presence of any significant acute or chronic, uncontrolled medical/ psychiatric illness 2. Hepatitis C virus antibody positive. 3. Human immunodeficiency virus antibody positive 4. History or evidence of autoimmune disease or known immunodeficiency of any cause 5. Prolonged therapy with immunomodulators (e.g. corticosteroids) or biologics (e.g. monoclonal antibodies, interferon) within 3 months of screening 6. Receipt of immunoglobulin or other blood products within 3 months prior to screening 7. Receipt of any investigational drug or vaccine within 3 months prior to screening 8. Cohorts 1-4: Receipt of any adenoviral vaccine within 3 months prior to administration of ChAdOx1-HBV on Day 0, or plan to receive an adenoviral-based vaccine within 3 months after Day 0 Cohorts 5 and 6: Receipt of any adenoviral vaccine (other than AZD1222 per inclusion criterion 13) within 3 months prior to administration of ChAdOx1-HBV on Day 0, or plan to receive an adenoviral-based vaccine within 3 months after Day 0 9. Receipt of any live vaccines within 30 days prior to screening 10. Receipt of any inactivated vaccines within 14 days prior to screening 11. History of allergic disease or reactions likely to be exacerbated by any component of the vaccine 12. Any history of anaphylaxis in reaction to vaccination 13. Malignancy within 5 years prior to screening with the exception of specific cancers that are cured by surgical resection (e.g. except basal cell skin carcinoma of the skin and cervical carcinoma). Participants under evaluation for possible malignancy are not eligible 14. Current alcohol or substance abuse judged by the Investigator to potentially interfere with participant safety and compliance 15. Significant cardiac disease or unstable uncontrolled cardiac disease 16. Any laboratory test at screening which is abnormal and which is deemed by the Investigator to be clinically significant 17. Any other finding that, in the opinion of the Investigator, deems the participant unsuitable for the study Additionally, for healthy participants (cohorts 1, 2, 5 and 6) 18. HBsAg positive Additionally, for participants with well controlled CHB (cohorts 3 and 4) 19. Co infection with hepatitis delta 20. Documented cirrhosis or advanced fibrosis indicated by a liver biopsy within 6 months prior to screening. In the absence of an appropriate liver biopsy, either 1 of the following: * Screening Fibroscan with a result \>9 kPa within ≤6 months of screening or * Screening FibroTest \>0.48 and aspartate aminotransferase (AST) to platelet ratio index of \>1 In the event of discordant results between non-invasive methods, the Fibroscan result will take precedence. 21. Alanine transaminase (ALT) \>3 × upper limit of normal, international normalised ratio (INR) \>1.5 unless the participant was stable on an anticoagulant regimen affecting INR, albumin \<35 g/L, total bilirubin \>2 mg/dL, platelet count \<100,000/mL 22. A history of liver decompensation (e.g. ascites, encephalopathy or variceal haemorrhage) 23. Prior or current hepatocellular carcinoma 24. Chronic liver disease of a non HBV aetiology 25. Any herbal supplements and or other medicines with potential liver toxicity within the previous 3 months prior to enrolment into this study