Effect of Passive Smoking on Oxidative Stress and Dental Caries in Children

Merve Erkmen Almaz180 enrolled

Overview

The purpose of the present study was to determine the effect of passive smoking on oxidative stress and dental caries in children.A total of 180 children were included to the study as follows; 90 children exposed to passive smoking, and 90 children in the control group (unexposed controls). Also demographic data were recorded (age, gender, parental education levels, child's tooth-brushing habit and child's daily dietary sugar exposure, family income). Dental examination of children were performed and caries prevalence of the patients were recorded. Unstimulated saliva samples were collected from children. Saliva 'cotinine levels' and oxidative stress markers 'total antioxidant status' (TAS), and' total oxidant status' (TOS) were evaluated by using Enzyme-linked immunosorbent assay (ELISA). The obtained data parameters of the two groups were evaluated and comparison was performed.

Study Type

OBSERVATIONAL

Enrollment

180

Conditions

Oxidative Stress Dental Caries Passive Smoking

Interventions

Eligibility

Sex: ALLMin age: 6 YearsMax age: 12 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: -Systemically healthy children Exclusion Criteria: * Uncooperative children * The presence of orthodontic/intraoral appliance * Any disease or condition known to affect oxidative stress

Outcomes

Primary Outcomes

determining oxidative stress marker in saliva: TAS

Unstimulated saliva samples were collected from children.

Time frame: 1 day (once after the dental examination)]

determining oxidative stress marker in saliva: TOS

Unstimulated saliva samples were collected from children.

Time frame: 1 day (once after the dental examination)

determining caries prevalence

Children were examined to determine caries prevalence.

Time frame: 1 day (once for dental examination)