Diagnostic Evaluation of Urine DNA Methylation/Somatic Mutation Profiling for the Detection of Urothelial Carcinoma

Inclusion Criteria: 1. Urothelial carcinoma group: * Any male or female patient aged 18 or older. * Able to provide urine specimen (100ml for both first void and non-first-void urine) before treatments. * Diagnosed with incident or recurrent urothelial carcinoma (including bladder/ureter/renal pelvis) by surgery. 2. interference group: * Any male or female patient aged 18 or older. * Able to provide urine specimen (100ml for both first void and non-first-void urine) before treatments. * Diagnosed with incident or recurrent bladder cancer other than urothelial carcinoma (including bladder squamous cell carcinoma/bladder adenocarcinoma/other bladder-related cancers/prostate cancer/rectal cancer) by surgery. 3. Control group: * Any male or female patient aged 18 or older. * Able to provide urine specimen (100ml for both first void and non-first-void urine) before treatments. * Diagnosed with urinary disease such as Urinary calculi, urinary tract infection (except urinary tuberculosis), benign prostatic hyperplasia, glandular cystitis. All enrollee are able to provide legally effective informed consent. 4. Healthy volunteers group: * Any male or female patient aged 18 or older. * Able to provide urine specimen (100ml for both first void and non-first-void urine) before physical examination. exclusion criteria: 1. Urothelial carcinoma/Interference group/Control group * Has had diagnosed with other cancers. * Patients diagnosed with non-urological cancer. * Failed to provide a written informed consent. 2. Healthy volunteers group: * Volunteers with abnormal test results of urine analysis or urological ultrasound test. * Volunteers sceptical of cancers from non-urological origin in a normal results of urine analysis or urological ultrasound. * Failed to provide legally effective informed consent.