The Effect of Platelet-Rich Plasma-Fibrin Glue in Combination With Vitamin E and C for Treatment of Non-healing Diabetic Foot Ulcers

Inclusion Criteria: 1. DFU is classified as Wagner 1 -2 on the Wagner classification system. 2. Persons with type 1 or type 2 diabetes with a non-healing ulcer of at least 4 weeks duration. 3. If more than one non-healing wound is present, the largest of the wounds that is classified as a Wagner 1- 2. 4. The index ulcer had to be clinically non-infected. 5. Ankle Brachial Index (ABI) greater than or equal to 0.7. 6. HemoglobinA1C (HbA1c) \< 12 7. Index foot ulcer located on the plantar, medial, or lateral aspect of the foot (including all toe surfaces). 8. Wound area (length x width) measurement between 2 cm2 and 20 cm2. 9. Non-use of drugs that may interfere with wound healing, such as corticosteroids, immunosuppressive agents, and cytotoxic agents. 10. No smoking, alcohol or drug addiction. 11. Approved, informed, signed consent. Exclusion Criteria: 1. Previous consumption of vitamin E and C supplements. 2. Index ulcer has exposed tendons, ligaments, muscle, or bone. 3. Confirmed presence of osteomyelitis, or if osteomyelitis is suspected. 4. Patients with renal deficiency, hepatic failure, has known immune insufficiency and cancer. 5. Subject is pregnant or plans to become pregnant during the duration of the trial. 6. Patient's blood vessels are non-compressible for ABI testing. 7. Patient is known to have a psychological, developmental, physical, emotional disorders. 8. Received systemic corticosteroids or immunosuppressive agents.