Netosis in Determination of Respiratory Infection Severity

Assistance Publique - Hôpitaux de Paris80 enrolled

Overview

The study aims to evaluate the prognostic value of alveolar and blood NETosis in patients under mechanical ventilation and treated for an acute low-respiratory tract infection. The main outcome is the occurrence of an acute respiratory distress syndrome (ARDS) according to the Berlin definition.

Neutrophils are the first line of defense against infectious injury. Among the numerous mechanisms involving the immune system, neutrophil extracellular traps (NETs) have been recently described as an additional way neutrophils are able to use to fight against bacteria. NETs are made of DNA and antimicrobial proteins. In the other hand, NETs promote coagulation and may contribute to evolution of severe pneumonia into acute respiratory distress syndrome. The secondary objectives of the study include the research of the relation between blood NETosis and alveolar NETosis with: * the documentation of invasive pulmonary infection based on microbiological analysis criteria (which would be bacteria or viral) of LBA at admission to ICU; * the diagnosis value of usually used biomarkers: procalcitonin and C-reactive protein; * marbrure score at admission to ICU; * arterial lactatemia; * scores of gravity at admission IGSII and SOFA; * all-cause mortality at day-28; * duration of mechanical ventilation at day-28; * duration of amines at day-28; * developement of hemodynamic failure; * developement of pulmonary circulatory failure. The study will include 1) 60 patients under mechanical ventilation admitted to the intensive care unit for acute respiratory failure, 2) 10 control patients admitted to the ICU after a planned vascular surgery and 3) 10 healthy subjects.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Respiratory Infection

Interventions

Blood samplingBIOLOGICAL

Blood sampling at baseline and at day-3 (10 ml, EDTA tube), test of NETs.

Bronchoalveolar lavagePROCEDURE

Bronchoalveolar lavage at admission to the ICU with analysis of cytological, bacteriological, viral and anatomopathological, and test of NETs.

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion criteria: A/ For all subjets: * Be \>/= 18 years of age on day of signing informed consent; * Affiliated to the french social security - welfare system in France. B/ Inclusion criteria for acute respiratory failure patients: admitted to the ICU for acute respiratory failure requiring mechanical ventilation within the first 24 hours after admission and suspected to suffer from an infectious pneumonia, according to the following criteria: * recent fever; * acute respiratory failure signs (dyspnea, polypnea \> 30 cycles/min, hypoxemia PaO2\<65 mmHg); * pulmonary focal signs of auscultation; * evocative signs in chest radiography or chest TDM. C/ Inclusion criteria for control patients: \- admitted to the ICU for post-operative management of vascular surgery. Exclusion Criteria: A/ For all subjets: * pregnancy, * nosocomial pneumonia, * no social health insurance, * neutropenia of any cause; * patient refusal. B/ Exclusion criteria for acute respiratory failure patients: \- no mechanical ventilation within the first 24 hours after admission to the ICU.

Locations (1)

Service de médecine intensive réanimation, Hôpital Ambroise Paré, APHP

Boulogne-Billancourt, France

Outcomes

Primary Outcomes

Acute respiratory distress syndrome occurrence

Acute respiratory distress syndrome occurrence according to the Berlin definition within the first 7 days after ICU admission

Time frame: at day-7

Secondary Outcomes

Mortality

Vital statue of patient

Time frame: At day-28

Duration of mechanical ventilation

Duration of mechanical ventilation: number of days without mechanical ventilation.

Time frame: At day-28

IGSII score

Time frame: At admission to ICU

SOFA score

Time frame: At admission to ICU

Hemodynamic failure

Development of a hemodynamic failure

Time frame: At day-28

Pulmonary circulation failure

Development of a pulmonary circulation failure (acute pulmonary heart disease, right ventricle failure)

Time frame: At day-28

Data from ClinicalTrials.gov

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