Muscle Response to High Intensity Interval Resistance Training (HIIRT)

University of Padova9 enrolled

Overview

Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.

Nine young healthy subjects performed HIIRT and TRT protocol in two different moments and with different legs. HIIRT technique consisted of three sets of: 6 repetitions at 6RM (6 repetitions maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. Biopsies from the vastus lateralis were taken at baseline (pre), immediately (0h) at the end of training, 6 hours (6h) and 24 hours (24h) after training. Western blot and RT-PCR mRNA (Reverse transcriptase-polymerase chain reaction) analysis were performed to assess muscle signalling pathway activation

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

SINGLE

Enrollment

Conditions

Exercise Resistance Training

Interventions

High Intensity Interval Resistance TrainingOTHER

Subjects trained one leg with HIIRT

Traditional Resistance TrainingOTHER

Subjects trained the other leg with HIIRT

Eligibility

Sex: ALLMin age: 20 YearsMax age: 30 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * moderate active * experience with resistance training Exclusion Criteria: * history of recent myocardial infarction, * severe cardiac arrhythmia, * unstable angina, * poorly controlled hypertension, * poorly controlled diabetes mellitus, * frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols

Locations (1)

Nutrition and Exercise Lab, DSB, University of Padova

Padua, Italy

Outcomes

Primary Outcomes

pAMPKThr172 in skeletal muscle tissue measured by immunoblot

measurement of pAMPKThr172 in skeletal muscle tissue taken by biopsy

Time frame: 24 hours

pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot

measurement of pERK 1/2Thr202/Tyr204 in skeletal muscle tissue taken by biopsy

Time frame: 24 hours

pS6Ser235/236 in skeletal muscle tissue measured by immunoblot

measurement of pS6Ser235/236 in skeletal muscle tissue taken by biopsy

Time frame: 24 hours

p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot

measurement of p4EBP1Thr37/46 in skeletal muscle tissue taken by biopsy

Time frame: 24 hours

ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot

ACC phosphorylation in skeletal muscle tissue taken by biopsy

Time frame: 24 hours

IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

IGF-1 mRNA in skeletal muscle tissue taken by biopsy

Time frame: 24 hours

IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

IGF-1ea mRNA in skeletal muscle tissue taken by biopsy

Time frame: 24 hoursn

MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

Data from ClinicalTrials.gov

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