Pseudomonas aeruginosa is a pathogenic bacteria for human, especially in hospital settings. It can sometimes be multi-resistant to many or even to all antibiotics usually used for its treatment. The aim of the study is to isolate and produce therapeutic antibodies against the bacteria Pseudomonas aeruginosa in order to provide an alternative treatment to antibiotics in case of infection with an antibiotic-resistant strain of Pseudomonas aeruginosa.
Study Type
OBSERVATIONAL
Enrollment
30
Blood sampling. Isolation of mononuclear cells from human peripheral blood by density gradient centrifugation. Identification of functional anti-Pseudomonas antibodies by ELISA and cellular infection assays.
Chu Grenoble Alpes
Grenoble, France
RECRUITINGIsolation of recombinant human monoclonal antibodies against Pseudomonas aeruginosa
Detection of binding of recombinant antibodies to Pseudomonas aeruginosa target proteins by ELISA assay.
Time frame: 1 day
Isolation of functional antibodies against Pseudomonas aeruginosa
Percentage of inhibition of Pseudomonas aeruginosa infection by antibodies using an in vitro model of cell infection and virulence. Percentage of lysis of Pseudomonas aeruginosa in the presence of recombinant IgG, complement and macrophages in an opsonophagocytosis assay
Time frame: 1 day
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