Accelerating Post-exercise Muscle Glycogen Resynthesis in Humans: Impact of Combined Nutrient Intake and Intermittent Pneumatic Compression or Heat Therapy

PepsiCo Global R&D26 enrolled

Overview

To determine if modalities designed to improve blood flow combined with post-exercise nutrient intake will improve replenishment of muscle glycogen better than nutrient intake alone. A secondary objective is to compare the effectiveness of heat therapy and intermittent pneumatic compression on glycogen replenishment.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Muscle Recovery Muscle Soreness Glycogen Depletion

Interventions

Compression sleeveDEVICE

From foot to hip/groin on both legs with only 1 leg connected to pneumatic pump for 1 hour

Heat therapyDEVICE

Custom leg garment with one leg heated by circulating hot water approx 40 degrees C, the other leg approx 32 degrees C (thermoneutral) for 1 hour

Glycogen-depleting exercise sessionOTHER

Prolonged intermittent high intensity running (LIST protocol)

Eligibility

Sex: ALLMin age: 18 YearsMax age: 35 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: 1. Subjects will be male and female participants in good health 2. 18-35 years of age 3. Currently performing cardiovascular or intermittent exercise at a moderate to high intensity at least 5 days per week for 60 minutes or longer per day. 4. Understanding of the procedures to be undertaken as part of the study 5. Willingness to participate in exercise testing and follow instructions provided by the experimenter 6. Informed, voluntary, written consent to participate in the study Exclusion Criteria: 1. History of deep vein thrombosis 2. Obesity (BMI \> 30 kg/m2) 3. Hypertension (resting SBP \> 140/90 mmHg) 4. Smoking 5. Pregnant, planning to become pregnant, or lactating 6. Supplements containing antioxidants including vitamin C and E and N-acetyl cysteine 7. Any medication 8. Lactose intolerance 9. Participation in another study within the previous 30 days or in a PepsiCo funded study within the past 6 months.

Locations (1)

Purdue University, Department of Health & Kinesiology

West Lafayette, Indiana, United States

Outcomes

Primary Outcomes

Rate of glycogen resynthesis

Leg skeletal muscle glycogen level assessment using assay kit

Time frame: Change from baseline to 1.5 hrs and 3.5 hrs post exercise bout. Biopsy 1 post-exercise pre nutrient intake 1 and compression (IPC) or heat, biopsy 2 post compression or heat, pre-nutrition intake 2, biopsy 3 at 210 min. Higher rate is better.

Muscle functional recovery: Skeletal muscle function

Work fatigue (%) of the knee extensors measured using an isokinetic dynamometer

Time frame: Change from pre-exercise to post-exercise and prior to biopsies and treatments, and 24 hours post exercise. Less fatigue is better.

Muscle functional recovery: Perceived muscle soreness

Knee extensor muscle soreness on a visual analog scale (VAS) of 100 mm after stepping on and off a 40 cm (female) or 45 cm (male) box three times

Time frame: Change from pre-exercise to post-exercise and prior to biopsies and treatments, and 24 hours post exercise. Less soreness is better.

Secondary Outcomes

mRNA expression of genes involved in regulation of glucose and glycogen metabolism and muscle functional recovery

Leg skeletal muscle fiber analysis determined using RT-PCR (Real-time polymerase chain reaction)

Time frame: Change from baseline to 1.5 hours and 3.5 hours post exercise bout. Biopsy 1 post-exercise pre- nutrient intake 1 and compression (IPC) or heat, biopsy 2 post compression or heat and pre- nutrition intake 2, biopsy 3 at 210 min. Higher expression better.

Serum insulin

Blood test using ELISA kit

Time frame: Change from post exercise at same time as biopsy 1 (Time 0), biopsy 2 (1.5 hrs) and biopsy 3 (3.5 hrs)

Serum Glucose

Data from ClinicalTrials.gov

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