This study aims for characterization of different maternal, infant and environmental factors (e.g. maternal age, pregnancy duration, lactation period, baby gender, circadian rhythm, Holder pasteurisation, etc.) that influence human milk composition.
The study consists of three parts. In the first part the investigators aim to evaluate human milk macronutrient composition depending on the time after delivery and pregnancy duration. In the second part the investigators aim to evaluate the circadian variation of human milk macronutrient and energy content depending on pregnancy duration. In the third part the investigators aim to evaluate the influence of Holder pasteurisation on human milk macronutrient, metabolome and bioactive protein (lysozyme and lactoferrin) content.
Study Type
OBSERVATIONAL
Enrollment
174
Analysis of human milk macronutrient content was performed by mid-infrared spectrophotometry using a Miris Human Milk Analyser. An immune-enzymatic ELISA assays were used to estimate lysozyme and lactoferrin concentrations in human milk.
Neonatal centre of Vilnius University Hospital Santaros Klinikos
Vilnius, Lithuania
Quantification of total protein concentration in human milk
Mid-infrared spectrophotometry
Time frame: from the 2nd week, up to 2 months after delivery
Quantification of total lipids and carbohydrate in human milk
Mid-infrared spectrophotometry
Time frame: from the 2nd week, up to 2 months after delivery
Quantification of lactoferrin in human milk
An immune-enzymatic ELISA assay
Time frame: 14-16 days after delivery
Quantification of lysozyme in human milk
An immune-enzymatic ELISA assay
Time frame: 14-16 days after delivery
Human milk metabolome analysis
Nuclear Magnetic Resonance (NMR) analysis
Time frame: 15-17 days after delivery
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