Crossover Study to Compare the Pharmacokinetics and Bioavailability of a Novel Furosemide Regimen Administered Subcutaneously vs. the Same Dose Administered Intravenously in Subjects With Chronic Heart Failure

Inclusion Criteria: * An Institutional Review Board (IRB)-approved informed consent is signed and dated prior to any study-related activities. * Male and female subjects ≥18 and ≤ 80 years of age, with body weight \<130 kg and body mass index (BMI) \<38 kg/m2. * Females will be non-pregnant, non-lactating, or post-menopausal, or surgically sterile (e.g., tubal ligation, hysterectomy), * Females of childbearing potential will use TWO of the following forms of contraception: intrauterine device (IUD), IUD with spermicide, female condom with spermicide, contraceptive sponge with spermicide, an intravaginal system, diaphragm with spermicide, cervical cap with spermicide, a male sexual partner who agrees to use a male condom with spermicide, a sterile sexual partner. * History of at least 3 months treated heart failure (NYHA class II/III) with presence of symptoms of chronic volume overload requiring ongoing treatment with oral furosemide at a dose of ≥40 mg per day for at least 30 days prior to Day -1. * Agrees to abstain from using alcohol, caffeine-containing products, and tobacco-/nicotine-containing products while in residence at the CRU. * Able to participate in the study in the opinion of the Investigator. * Has the ability to understand the requirements of the study and is willing to comply with all study procedures. Exclusion Criteria: * Acute Decompensated Heart Failure (ADHF) or recent history of hospitalization for heart failure in the last 4 weeks. * Worsening of signs or symptoms of heart failure in the two weeks prior to the Screening, or those expected to require IV loop diuretics or inpatient treatment for heart failure during the study. * Systolic blood pressure (SBP) \<90 mmHg. * Temperature ≥38°C (oral or equivalent) or sepsis or active infection requiring IV anti-microbial treatment. * Serum sodium \<130 mEq/L and serum potassium \<3.5 mEq/L. * Significant other cardiac abnormalities which may interfere with study participation or study assessments. * Current or planned treatment during the study with any IV therapies, including inotropic agents, vasopressors, levosimendan, nesiritide or analogues; or mechanical support (intraaortic balloon pump, endotracheal intubation, mechanical ventilation, or any ventricular assist device). * Subject is cachectic. * Diagnosed with Type I diabetes mellitus or Type II diabetes requiring insulin therapy. * Presence or need for urinary catheterization, urinary tract abnormality, or disorder interfering with urination. * Impaired renal function, defined as an estimated glomerular filtration rate (eGFR) on admission \<45 mL/min/1.73m2, calculated using the simplified Modification of Diet in Renal Disease (sMDRD) equation. * Indication of moderate-to-severe hepatic dysfunctions as determined by the Investigator. * Administration of IV radiographic contrast agent within 72 hours prior to Screening or acute contrast-induced nephropathy at the time of Screening. * Major surgery within 30 days prior to Screening. * Administration of an investigational drug or implantation of investigational device, or participation in another interventional trial, within 30 days prior to Screening. * Any surgical or medical condition, which in the opinion of the Investigator may pose an undue risk to the subject, interfere with participation in the study, or which may affect the integrity of the study data. * Positive test for hepatitis B (HBsAg), hepatitis C (HCV), or human immunodeficiency virus (HIV) at Screening. * Any positive urine drug screen at Screening or clinic admission. * Concomitant use of any drugs known to interact with furosemide. * History of alcohol abuse within 6 months prior to Screening and/or signs or symptoms of alcoholism, as determined by the Investigator. * Any positive alcohol test on admission to the CRU. * History of severe allergic or hypersensitivity reactions to furosemide. * Donation of greater than 100 mL of either whole blood or plasma within 30 days prior to study drug administration. * Been informed of possible COVID-19 exposure in past 4 weeks, or recent onset of signs or symptoms of possible COVID-19 infection, including cough, shortness of breath, or temperature ≥ 38°C . * Traveled via airplane or cruiseship within the last 14 days.