Efficacy of Combined Blood Clot and Platelet Rich Fibrin Scaffolds in Regeneration of Necrotic Immature Permanent Teeth

Nourhan M.Aly30 enrolled

Overview

The aim of the present study is to assess clinically and radio-graphically the regenerative potential of immature permanent teeth with necrotic pulp using blood clot and Platelet rich fibrin scaffolds.

30 immature necrotic permanent teeth in patients will be allocated and divided into two groups. Group 1 will be treated by regenerative procedure using blood clot as a scaffold and Group 2 will be treated with regenerative procedures using combined blood clot and Platelet rich fibrin as a scaffold.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

TREATMENT

Masking

SINGLE

Enrollment

Conditions

Pulp Necroses

Interventions

Blood clot scaffoldBIOLOGICAL

Bleeding will be induced in the canal by passing a size 30 sterile K-file 3 mm beyond the apex with the goal of having the entire canal filled with blood to the level of the cemento-enamel junction. Bleeding will be stopped just below the cemento enamel junction. Biodentine will be placed approximately 3-4 mm below the cementoenamel junction.

Blood clot scaffold combined with PRFBIOLOGICAL

Platelet-rich fibrin will be prepared by drawing the patient blood into a 10mL test tube without the addition of an anticoagulant. To prevent the blood from coagulating after coming in contact with the glass tube, it will be centrifuged immediately using a table top centrifuge\*\* at 400 g force for 12 minutes. Then platelet-rich fibrin membrane will be placed into the canal space to a level 3 mm below the cemento-enamel junction using hand plugger following the induction of apical bleeding by passing a number 30 sterile hand file 3 mm beyond the apex of the tooth. Biodentine cap will be placed over the platelet-rich fibrin scaffold.

Eligibility

Sex: ALLMin age: 8 YearsMax age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Necrotic incisors with immature roots * Pulp space not needed for post and core. * The patient has to be free from any coagulation disorders, with normal platelets count. * Good oral hygiene * Cooperative patient Exclusion Criteria: * Internal or external root resorption. * Root fracture and/or alveolar fracture. * Previous root canal treatment. * Medically compromised patients * Uncooperative patient

Locations (1)

Faculty of Dentistry, Alexandria University

Alexandria, Egypt

Outcomes

Primary Outcomes

Subjective pain assessment

The patient will be asked about the presence of any pain or discomfort. Pain severity will be categorized as: no pain - mild pain - moderate pain - severe pain

Time frame: 1 month

Subjective pain assessment

The patient will be asked about the presence of any pain or discomfort. Pain severity will be categorized as: no pain - mild pain - moderate pain - severe pain

Time frame: 3 months

Subjective pain assessment

The patient will be asked about the presence of any pain or discomfort. Pain severity will be categorized as: no pain - mild pain - moderate pain - severe pain

Time frame: 6 months

Subjective pain assessment

The patient will be asked about the presence of any pain or discomfort. Pain severity will be categorized as: no pain - mild pain - moderate pain - severe pain

Time frame: 12 months

Assessment of pulp vitality

Pulp vitality will be assessed using thermal and electric pulp sensibility tests

Time frame: 1 month

Assessment of pulp vitality

Pulp vitality will be assessed using thermal and electric pulp sensibility tests

Time frame: 3 months

Assessment of pulp vitality

Pulp vitality will be assessed using thermal and electric pulp sensibility tests

Time frame: 6 months

Assessment of pulp vitality

Pulp vitality will be assessed using thermal and electric pulp sensibility tests

Data from ClinicalTrials.gov

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