The investigators aim to examine the endometrium with state of the art sequencing techniques to investigate the endometrial microbiota. The endometrial microbiota has been perceived to be sterile, however, this seems incorrect from recent studies. Thus, the primary outcome is to compare the rate of ascending infection from the vagina to the endometrium and to investigate which bacteria are capable of inhabiting these environments in healthy oocyte donors. These results will be compared to equivalent samples of IVF patients.
Study Type
OBSERVATIONAL
Enrollment
26
Instituto Bernabeu
Alicante, Spain
Rate of ascending infection/sharing of bacteria in the genital tract
vaginal, cervical and endometrial samples will be compared at the time of oocyte retrieval.
Time frame: This outcome data is measured at the 1 day of oocyte retrieval.
Next generation sequencing techniques and qPCR will be used to assess the microbiota.I.e. measure of relative abundances and absolute abundances
qPCR to assess the microbiota. I.e. a quantitative measure Copies/mL. 16S V4 to investigate relative abundances
Time frame: This outcome data is measured at the 1 day of oocyte retrieval.
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