Identification and Characterization of Novel Non-Coding Variants That Contribute to Genetic Disorders

Duke University56 enrolled

Overview

The goal of this study is to identify and characterize novel non-coding and splicing variants that may contribute to genetic disorders. We will particularly focus on patients with a diagnosed genetic disorder that has inconclusive genetic findings.

To perform this study, we will use patient DNA and RNA that is isolated from blood samples. DNA will be sequenced (targeted capture and/or whole genome DNA sequencing (WGS)) to identify any non-coding single nucleotide variants (SNVs), smaller insertions/deletions (indels), or larger structural variants (SVs). RNA will be sequenced (RNA-seq) to identify genes that are expressed in a differential and/or allele-specific manner, which may indicate a functional non-coding or splicing variant. We will test the function of non-coding variants using high-throughput reporter assays and CRISPR based methodologies.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Genetic Disease Inborn Errors of Metabolism Glycogen Storage Disease Lysosomal Storage Diseases Storage Disease

Eligibility

Sex: ALL

Medical Language ↔ Plain English

Inclusion criteria: Subjects will have one or more of the following: * Patients (probands) diagnosed with a genetic disease * Patients (probands) with inconclusive genetic results * Patients (probands) that have identical coding and/or splicing variants, but display highly diverse phenotypes * Unaffected family members of probands Exclusion Criteria: There are no exclusion criteria for this study.

Locations (1)

Duke University

Durham, North Carolina, United States

Outcomes

Primary Outcomes

Number of missing pathogenic protein coding variants

Time frame: 2 years

Data from ClinicalTrials.gov

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