The SARS-CoV-2 infection in the airway epithelium induces cytopathic effects and the cessation of ciliary movement. Increased cytokines and chemokines have been reported to be associated with the severity of the disease. However, most of the molecular and cellular aspects of the inflammatory response and the processes of development of humoral and cellular immunity in these patients are unknown. The aim of this study is characterizing inflammatory processes, seeking to expand the knowledge of the cellular and molecular pathophysiology of COVID-19 that could help in the decision-making of treating health personnel. Mainly, the study is focused on analyzing the inflammatory response by determining cytokines and chemokines. Also, the viral load of the patients with COVID-19 will be determined and will be correlated with the antibody titers. On the other hand, cells will be immunophenotyped to search the cellular depletion profile. Finally, an epidemiological analysis of the patients will be carried out.
Study Type
OBSERVATIONAL
Enrollment
200
Adult patients admitted to the UMAE Specialty Hospital of CMN SXXI with a diagnosis of COVID-19. The diagnosis must be corroborated by the RT-PCR test.
UMAE Hospital de Especialidades del Centro Médico Nacional Siglo XXI, IMSS.
Mexico City, Mexico City, Mexico
RECRUITINGSerum levels of cytokines and chemokines
Pg/ml of IL-2, CXCL8 / IL-8,IL-4, CCL5 / RANTES, IL-6, CXCL9 / MIG, IL-10, CCL2 / MCP-1, TNF-alfa, CXCL10 / IP-10, IFN-alfa, soluble receptor IL6, IFN-gamma.
Time frame: Day 0
Serum levels of cytokines and chemokines
Pg/ml of IL-2, CXCL8 / IL-8,IL-4, CCL5 / RANTES, IL-6, CXCL9 / MIG, IL-10, CCL2 / MCP-1, TNF-alfa, CXCL10 / IP-10, IFN-alfa, soluble receptor IL6, IFN-gamma.
Time frame: Day 3
Serum levels of cytokines and chemokines
Pg/ml of IL-2, CXCL8 / IL-8,IL-4, CCL5 / RANTES, IL-6, CXCL9 / MIG, IL-10, CCL2 / MCP-1, TNF-alfa, CXCL10 / IP-10, IFN-alfa, soluble receptor IL6, IFN-gamma.
Time frame: Day 7
Viral load
log10 U/ml
Time frame: Day 0
Viral load
log10 U/ml
Time frame: Day 7
Immunophenotype of myeloid cells
Immunophenotypic profile of myeloid cells in COVID by surface molecules CD45, CD14, CD16, mIL-6R, CD73 and CD3. reported in relative fluorescence units and relative percentage of cells analyzed by flow cytometry
Time frame: Day 0
Immunophenotype of myeloid cells
Immunophenotypic profile of myeloid cells in COVID by surface molecules CD45, CD14, CD16, mIL-6R, CD73 and CD3. reported in relative fluorescence units and relative percentage of cells analyzed by flow cytometry
Time frame: Day 3
Immunophenotype of myeloid cells
Immunophenotypic profile of myeloid cells in COVID by surface molecules CD45, CD14, CD16, mIL-6R, CD73 and CD3. reported in relative fluorescence units and relative percentage of cells analyzed by flow cytometry
Time frame: Day 7
RBD-SARS-CoV Protein S- antibodies
relative absorbance units by ELISA
Time frame: Day 0
RBD-SARS-CoV Protein S- antibodies
relative absorbance units by ELISA
Time frame: Day 3
RBD-SARS-CoV Protein S- antibodies
relative absorbance units by ELISA
Time frame: Day 7
Analysis of cellular immune response
Cell count per area (/ mm2) of immune cell sub-populations
Time frame: Day 0
Analysis of cellular immune response
Cell count per area (/ mm2) of immune cell sub-populations
Time frame: Day 3
Analysis of cellular immune response
Cell count per area (/ mm2) of immune cell sub-populations
Time frame: Day 7
Hematopoietic stem cells and progenitor cells populations in peripheral blood
Immunophenotypic profile of stem cells and progenitor cells populations COVID by surface molecules CD34, CD38, CD45RA, Lin, CD3, CD8, CD56, CD19, CD20, CD11b, CD235a andCD14. reported in relative fluorescence units and relative percentage of cells, analyzed by flow cytometry
Time frame: Day 0
Hematopoietic stem cells and progenitor cells populations in peripheral blood
Immunophenotypic profile of stem cells and progenitor cells populations COVID by surface molecules CD34, CD38, CD45RA, Lin, CD3, CD8, CD56, CD19, CD20, CD11b, CD235a andCD14. reported in relative fluorescence units and relative percentage of cells, analyzed by flow cytometry
Time frame: Day 3
Hematopoietic stem cells and progenitor cells populations in peripheral blood
Immunophenotypic profile of stem cells and progenitor cells populations COVID by surface molecules CD34, CD38, CD45RA, Lin, CD3, CD8, CD56, CD19, CD20, CD11b, CD235a andCD14. reported in relative fluorescence units and relative percentage of cells, analyzed by flow cytometry
Time frame: Day 7
Questionnaire of Sociodemographic, labor, pathological and personal characteristics
Questionnaire
Time frame: Day 0
SOFA (Secuential Organ Failure Assessment Score)
Is used to track a person's status during the stay in an intensive care unit to determine the extent of a person's organ function or rate of failure. The score is based on six different scores, one each for the respiratory, cardiovascular, hepatic, coagulation, renal and neurological systems.
Time frame: Day 0
SOFA (Secuential Organ Failure Assessment Score)
Is used to track a person's status during the stay in an intensive care unit to determine the extent of a person's organ function or rate of failure. The score is based on six different scores, one each for the respiratory, cardiovascular, hepatic, coagulation, renal and neurological systems.
Time frame: Day 3
SOFA (Secuential Organ Failure Assessment Score)
Is used to track a person's status during the stay in an intensive care unit to determine the extent of a person's organ function or rate of failure. The score is based on six different scores, one each for the respiratory, cardiovascular, hepatic, coagulation, renal and neurological systems.
Time frame: Day 7
Fibrinogen
mg/dL
Time frame: Day 0
Fibrinogen
mg/dL
Time frame: Day 3
Fibrinogen
mg/dL
Time frame: Day 7
C-Reactive protein
mg/L
Time frame: Day 0
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C-Reactive protein
mg/L
Time frame: Day 3
C-Reactive protein
mg/L
Time frame: Day 7