Perforated diverticulitis of the colon can be complicated by peritonitis, either purulent or fecal. Both are life threatening conditions requiring emergency surgery. The background in terms of reasons for inflammation (diverticulitis) and perforation to develop is unknown. In this study we focus on specific changes associated with inflammation, both in tissues and of fecal and mucosal microbiota.
A prospective study of abdominal fluid, periferal blood, tissues as well as feces in Patients (perforated diverticulitis with peritonitis undergoing emergency surgery, Group I), and in Controls (patients operated due to colorectal cancer, Group II; patients with mild diverticulitis not needing surgery, Group III). Immune acitvity in abdominal fluid and periferal blood by immunoassay of 92 individual proteins. mRNA expression of 96 specific genes in tissue samples. Quatification of bacterial diversity in feces and colon mucosa.
Study Type
OBSERVATIONAL
Enrollment
50
Sahlgrenska University Hospital/Östra
Gothenburg, Västra Götaland County, Sweden
RECRUITINGinflammatory response
determination of levels of 92 proteins in abdominal fluid and blood
Time frame: index surgery (Groups I and II)/ emergency room (Group III)
inflammatory response in tissue
RNA sequencing (96 spcific genes) in abdominal tissue
Time frame: index surgery (Groups I and II)/ emergency room (Group III)
microbiota composition
PCR amplification, cloning and sequencing of genes
Time frame: index surgery (Groups I and II)/ emergency room (Group III)
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