Assessment of Progranulin and FAM19A5 Protein Blood Levels in Metabolic Syndrome

Poznan University of Medical Sciences80 enrolled

Overview

The main objective of the study is to assess the serum levels of progranulin and FAM19A5 protein in adults with metabolic syndrome.

The study is planned to be conducted on 40 adults with metabolic syndrome. As a control, it is planned to recruit 40 adults without metabolic syndrome. From all participants fasting venous blood samples, urine samples and hair samples will be collected. Next, serum progranulin and serum FAM19A5 protein concentrations will be determined in both groups. Also, serum concentration of leptin, vascular endothelial growth factor (VEGF), insulin, neopterin, adropin and selected cytokines, adipokines, myokines and chemokines, total cholesterol, low-density lipoproteins, high-density lipoproteins, triglycerides and glucose will be determined. Serum, hair and urine mineral content will be determined. In whole blood genetic analyses will be performed in order to detect and examine progranulin and FAM19A5 genes, and genes associated with the development of metabolic syndrome and genes associated with the body's mineral metabolism. Also, body composition analysis, blood pressure measurement, pulse measurement, blood oxygen saturation measurement and nutritional interview will be performed.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Nutrition Disorders Hypertension Dyslipidemias Glucose Intolerance Glucose Metabolism Disorders Obesity Overweight and Obesity Metabolic Syndrome

Interventions

Blood sample collectionDIAGNOSTIC_TEST

Fasting blood sample will be collected from forearm vein. In the sample the concentration of: progranulin and serum FAM19A5 protein, leptin, VEGF, insulin, neopterin, adropin and selected cytokines, adipokines, myokines and chemokines, total cholesterol, low-density lipoproteins, high-density lipoproteins, triglycerides and glucose will be determined. Whole blood sample will be secured to isolate genetic material, detect and examine progranulin and FAM19A5 genes, and genes associated with the development of metabolic syndrome and with the body's mineral management. Serum mineral analysis will be performed.

Hair sample collectionDIAGNOSTIC_TEST

Hair sample collection will be performed. Hair mineral analysis will be performed.

Urine sample collectionDIAGNOSTIC_TEST

Eligibility

Sex: ALLMin age: 18 YearsMax age: 99 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * written and informed consent of the participant to participate in the study; * age 18-99; * meeting at least three of the following five metabolic syndrome criteria: * waist circumference ≥80 cm in women and ≥94 cm in men; * serum triglycerides\> 150 mg / dl (1.7 mmol / l) or treatment of hypertriglyceridemia; * HDL serum concentration \<50 mg / dl (1.3 mmol / l) - in women and \<40 mg / dl (1.0 mmol / l) - in men or treatment of this lipid disorder; * systolic blood pressure ≥ 130 mm Hg or diastolic blood pressure ≥ 85 mm Hg, or treatment of previously diagnosed hypertension; * fasting plasma glucose ≥100 mg / dl (5.6 mmol / l) or pharmacological treatment of type 2 diabetes. Exclusion Criteria: * secondary form obesity, * substitution of progranulin or / and FAM19A5 protein, * weight reduction above 5% of baseline body weight in the last 3 months preceding recruitment, * liposuction and / or other fat reduction treatments, * pacemaker, cardioverter / defibrillator, * condition after stroke, * Alzheimer's disease, * fronto-temporal dementia, * occurrence of other neurodegenerative diseases, * occurrence of significant neurological disorders, * occurrence of inflammatory autoimmune diseases, * lysosomal storage diseases, * clinically significant abnormal liver, kidney or thyroid function; * acute or clinically significant inflammatory process in the respiratory, digestive, genitourinary tracts, * connective tissue diseases or arthritis; * active cancer, * addiction to alcohol or drugs; * pregnancy or childbirth during recruitment or 3 months before recruitment; * current lactation or lactation within 3 months before recruitment; * and / or any other condition which, according to researchers, would cause that participation would not be in the patient's best interest or could limit the credibility of the study

Locations (1)

Department of Treatment of Obesity, Metabolic Disorders and Clinical Dietetics, Poznan University of Medical Sciences

Poznan, Wielkopolska, Poland

RECRUITING