Gingival Crevicular Fluid Alkaline Phosphatase as Skeletal Growth Indicator

Inclusion Criteria: 1. Gender: only female subjects will be included. 2. Age between 7-16 years old 3. Intermediate or late mixed dentition and permanent dentition 4. Good general health with absence of any nutritional problems or bony disease 5. No usage of any anti-inflammatories or antibiotics in the month preceding the study 6. Fully erupted upper and lower central incisors for sample collection 7. Good gingival and periodontal health as indicated by Probing depth (PD) values that should not exceed 3 mm for the whole dentition. 8. Willingness to participate in the study and sign the informed consent form Exclusion Criteria: 1. Male adolescent subjects 2. Periodontal and /or gingival disease 3. Systemic and/or bone disease 3\. Growth abnormality