Adipokines, Inflammation, Insulin Resistance and Endothelial Dysfunction in Preeclampsia

Tanta University60 enrolled

Overview

The aim of the present study is to evaluate the implication of adipokines, inflammation, insulin resistance and endothelial dysfunction in the pathogenesis of preeclampsia and in pregnancy related complications.

This observational parallel study will be conducted on pregnant women with normal pregnancy, pregnant women with Preeclampsia, and on healthy non-pregnant women. All participants will be recruited from the Outpatient Clinic of Obstetrics and Gynecology Department at Menoufia University Hospital (Shebin El-Kom, Egypt). The study will be approved by the Research Ethics Committee of Menoufia University and all the involved subjects will give their written informed consent. This study will involve a total number of 60 women who will be further classified into: 20 non-pregnant women who serve as the control group (group 1), 20 pregnant women with normal pregnancy at their third trimesters (group 2) and 20 pregnant women with Preeclampsia at their third trimester (group 3) will be screened to fit the inclusion and exclusion criteria. The pregnant women will be assessed in their third trimesters and further re-evaluated 4 weeks after delivery. The gestational ages will be determined conventionally and re-affirmed by ultrasonographic measurement.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Pre-Eclampsia

Interventions

Serum asymmetteric dimethyl arginineDIAGNOSTIC_TEST

Serum asymmetteric dimethyl arginine (ADMA) will be done using Enzyme-Linked Immunosorbent Assay

serum lipid profilesDIAGNOSTIC_TEST

serum lipid profiles including triglycerides (TG), total cholesterol and high-density lipoprotein cholesterol (HDL-C) which will be measured by enzymatic colorimetric method) using commercial kits

Serum vaspinDIAGNOSTIC_TEST

vaspin concentrations will be assayed using commercially available ELISA kits

Eligibility

Sex: FEMALEMin age: 25 YearsMax age: 35 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Pregnant women with normal pregnancy (n=20) * Pregnant women with Preeclampsia (n=20) at their third trimesters of pregnancy will be included in the study. Preeclampsia will be defined and diagnosed by constant elevation in blood pressure greater than 140/90 mmHg and by the presence of proteinuria above 300 mg /24 hour with the absence of both urinary tract infection and previous history of hypertension. * Apparently healthy non-pregnant females (n=20) will be included in this study also. Exclusion Criteria: * Women with risk factors for oxidative stress such as smokers and * Those with a medical history of diabetes mellitus (DM) and Tuberculosis (T.B). * Women with a history of hypertension, familial hyperlipidemia, liver diseases, inflammatory diseases, and renal impairment. * Non-pregnant women on hormonal therapy or hormonal contraceptives.

Outcomes

Primary Outcomes

Interleukin 18

IL-18 will be carried out using Enzyme-Linked Immunosorbent Assay

Time frame: Nine months

Vaspin level

Serum vaspin concentrations will be assayed using commercially available ELISA kits

Time frame: Nine months

resistin level

Serum resistin concentrations will be assayed using commercially available ELISA kits

Time frame: Nine months

Malondialdehyde level

MDA will be measured spectrophotometrically

Time frame: Nine Months

Serum asymmetteric dimethyl arginine (ADMA)

Serum asymmetteric dimethyl arginine (ADMA) will be done using Enzyme-Linked Immunosorbent Assay

Time frame: Nine Months

insulin resistance (IR)

insulin resistance (IR) using the HOMA-IR index \[18\], which is defined as fasting insulin (μIU/ml) times fasting glucose (mmol/l) divided by 22.5 or divided by 405 if fasting blood glucose is expressed in mass units (mg/dl).

Time frame: Nine Months

Data from ClinicalTrials.gov

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