laser-assisted ICSI (LA-ICSI) was described to be a new method combining a less invasive ICSI technique with assisted hatching.This procedure is usually used for MII oocytes possessing oolemma breakage difficulties, but why not using it for even normal Oocytes?!. Especially because data indicated that in addition to an improved oocyte survival, this new approach increases the hatching rate in vitro. But since it is still debatable that such a small opening might impair the hatching process, so modified zona thinning technique was proposed for more embryo safety.
This study aims to compare the LA- ICSI by drilling a micro hole (LAD-ICSI) and modified LA-ICSI by zona pellucida thinning (LAT-ICSI) with conventional ICSI in order to effectively assess whether this approach can be applied routinely to improve ICSI outcome. One arm study divided into three interventions; Conventional ICSI (control group) was compared with LAD-ICSI (Study group 1) and LAT- ICSI (study group 2) in sibling oocytes. The rates of maturation, fertilization, Devision, Blastulation and good quality blastulation rates were assessed in the three groups Cases were randomly selected for embryo transfer from the three groups,Then chemical pregnancy and clinical pregnancy rates were compared between the three groups.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
DOUBLE
Enrollment
120
using OCTICS laser device to do small opening or zona thinning to improve ICSI outcome
Royal Fertility Center
Al Mansurah, Dakahlia Governorate, Egypt
Clinical pregnancy rate
confirmed by sac observation using ultrasound
Time frame: three-four weeks after embryo transfer
Blastulation rate
number of blastocysts that are grown from devided embryos
Time frame: 5 days later after ICSI procedure
Hatching rate
number of blastocysts that hatch out of zona
Time frame: 5 days later after ICSI procedure
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.