The primary end-point of our prospective, observational study is to count T cells in patients with laboratory-confirmed COVID-19 and healthy controls. In addition, the expression of T cell exhaustion marker was measured in COVID-19 cases.
COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has posed great threat to human health. T cells play a critical role in antiviral immunity but their numbers and functional state in COVID-19 patients remain largely unclear. The immune response against viral infections depends on the activation of cytotoxic T cells that can clear infection by killing virus-infected cells, so boosting the numbers and function of T cells in COVID-19 patients is critical for successful recovery. However, the factors which might cause the reduction in count, and the activation status of T cells in COVID-19 patients, remain uninvestigated. Thus demonstration of T cell exhaustion during COVID-19 infection suggest that more urgent, early intervention may be required in patients with low T lymphocyte counts.
Study Type
OBSERVATIONAL
Enrollment
100
Lymphocyte subsets in peripheral blood were assessed by staining 50 µl of blood sample with 5 µl of Fluoroisothiocyanate (FITC)-conjugated- PD-1, phycoerythrin (PE)-conjugated-CD8, peridinium-chlorophyll-protein (Per-CP)-conjugated-CD4, Peridinium-chlorophyll-protein (Per-CP)-conjugated anti-CD3 and allophycocyanin (APC) conjugated anti-CD28.
Faculty of Medicine
Asyut, Egypt
RECRUITINGCD4+ (T-helper cells) in COVID 19 patients and healthy volunteers.
Detection of the percentage of CD4+ (T-helper cells) in COVID 19 patients and healthy volunteers.
Time frame: Baseline
CD8+ (T-cytotoxic cells) in COVID 19 patients and healthy volunteers.
Detection of the percentage of CD8+ (T-cytotoxic cells) in COVID 19 patients and healthy volunteers .
Time frame: Baseline
Detect PD-1 on CD8+ and CD4+ cells in COVID 19 patients and healthy volunteers .
Detection of the percentage of expression of PD-1 on CD8+ and CD4+ cells in COVID 19 patient and healthy volunteers.
Time frame: Baseline
Detect CD28+ and CD3+ cells in COVID 19 patients and healthy volunteers.
Detection of the frequency of CD28+ and CD3+ cells in COVID 19 patients and healthy volunteers .
Time frame: Baseline
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