Comparing the reproductive outcomes of different sperm selection techniques; Density gradient centrifugation (DGC), Testicular sperm (Testi), Physiological ICSI (PICSI), and Magnetic activated cell sorting (MACS) in abnormal sperm DNA fragmentation (SDF) ICSI patients.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
SINGLE
Enrollment
320
Semen processing is done by double layer density gradient method.
Sperm selection by PICSI dishes depending on hyaluronan binding of sperm head
Sperm selection by using Annexin V micro-beads and separation columns
Ganin Fertility Center
Cairo, Maadi, Egypt
Clinical pregnancy rate
Defined as clinical pregnancy per transfer
Time frame: 14 days following embryo transfer
Implantation rate
Defined as number of gestational sacs with fetal heart beat, shown by ultrasound in gestational week 6 over number of embryo transferred.
Time frame: 6- 8 weeks following embryo transfer
Ongoing pregnancy rate
Defined as the proportion of pregnancies that completed more than 20 weeks of gestation
Time frame: 20 weeks of gestation
Cleavage rate
Defined as the proportion of cleaved embryos on day 3 over the injected oocytes
Time frame: 3 days
Blastulation rate
Defined as the proportion of blastocysts formed on day 5 or 6 over the cleaved embryos on day 3
Time frame: 5-6 days
High quality blastocyst rate
Defined as the assessment of blastocyst quality according to Gardner's criteria into: good, fair or bad in terms of percentage of the total formed blastocysts
Time frame: 5-6 days
Miscarriage rate
Defined as the proportion of miscarried cases
Time frame: 20 weeks of gestation
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Using testicular sperm retrieved by TESA or TESE