A prospective randomized study included 1695 MII sibling oocytes collected from 100 patients undergoing ICSI.
Purpose: to determine the effect of culture media microdroplet geometry of different culture dishes and oil overlay on the pre implantation embryo development in dry incubators Method: We used sibling oocytes splits between two different dishes : GPS (life global, USA) and SPL (life science, Korea) with two different oils : light mineral oil (LM)(Irvine, USA) and paraffin oil (PO)(Vitro life, Sweden). All embryos cultured in dry incubators at the same conditions. statistical analysis done using SPSS version 23
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
NONE
Enrollment
100
Ganin Fertility Center
Cairo, Egypt
Blastulation rate
Percentage of total blastocysts / Total cleaved embryos
Time frame: 5-6 days after ICSI
High quality blast rate
Morphological grading of trophectoderm and Inner cell mass using Gardner's criteria 1999, from grade A and B ( A is the highest quality). Total high grade embryos/ total blast embryos.
Time frame: 5-6 days after ICSI
Fertilization rate
Percentage of fertilized oocyte (appearance of two pronuclei) / Number of injected oocytes
Time frame: 16-19 hours of ICSI
Cleavage rate
Percentage of cleaved embryos/ total number of fertilized oocytes
Time frame: 72 hours of ICSI
High quality D3 embryos
High quality D3 embryos according to Gardner's criteria / total cleaved embryos
Time frame: 72 hours of ICSI
Good quality trophectoderm/inner cell mass
better grade than 3BB of blast embryos according to Gardner's criteria 1999
Time frame: 5-6 days after ICSI
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Media microdroplet geometry (2D dishes) in combination with paraffin oil overlay