A prospective cohort study undergoing ICSI at a private fertility center in Egypt
Purpose: To evaluate PGTai results for embryos cultured in sequential versus single step media. Method: Injected oocytes will divided between Continuous Single Culture-NX media (CSC- NX) with 10% SSS the first group (Irvine, USA) and the other group will be cultured in sequential cleavage and blastocyst media with 10% SPS (SAGE, Denmark). All blastocytes with 4BB quality and better according to Gardner's criteria 1999 will go through PGTA procedures with PGTai confirmation.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
OTHER
Masking
NONE
Enrollment
200
Usage of single step media in embryo culture
Usage of sequential media in embryo culture
Ganin Fertility Center
Cairo, Egypt
RECRUITINGEuploidy rate
percentage of euploid embryos/ total genetic tested embryos on blast stage
Time frame: 5:7 days of intracytoplasmic sperm injection(ICSI)
Mosaicism rate
percentage of mosaic embryos/ total genetic tested embryos on blast stage
Time frame: 5:7 days of ICSI
Fertilization rate
Percentage of fertilized oocyte (appearance of two pronuclei) / Number of injected oocytes
Time frame: 16:19 hours of ICSI
Cleavage stage
Percentage of cleaved embryos/ total number of fertilized oocytes
Time frame: 72 hours of ICSI
High quality of cleaved embryos
High quality D3 embryos according to Gardner's criteria / total cleaved embryos
Time frame: 72 hours of ICSI
Blast formation rate
Percentage of total blastocysts / Total cleaved embryos
Time frame: 5:7 days of ICSI
High quality blast rate
Morphological grading of trophectoderm and Inner cell mass using Gardner's criteria 1999, from grade A and B ( A is the highest quality). Total high grade embryos/ total blast embryos.
Time frame: 5:7 days of ICSI
Amr Elshimy, BSc.
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