A Clinical Study to Develop a Controlled Human Infection Model Using Leishmania Major-infected Sand Flies

Inclusion Criteria: * Healthy adults aged 18 to 50 years on the day of screening * Willing to give consent for exposure to Leishmania-infected sand fly with the intention of causing a cutaneous leishmaniasis lesion * Willing and able to give written informed consent * Willing to undergo Hepatitis B, Hepatitis C \& HIV testing * Willing to undergo a pregnancy test during screening and follow-up visits and must not be breastfeeding * Willing to refrain from blood donation during the study * Using a reliable and effective form of contraception (pre-menopausal female participants) * Judged, in the opinion of a medically qualified Clinical Investigator, to be able and likely to comply with all study requirements as set out in the protocol * Without any other significant health problems as determined by medical history, physical examination, results of screening tests and the clinical judgment of a medically qualified Clinical Investigator * Available for the duration of the study * Willing to refrain from travel to regions where Leishmania-transmitting sand flies are present, from recruitment until an appropriate point (judged by study investigators). * Willing to consent to a copy of the past medical history to be provided by the participants GP (General Practitioner) practice. * Agree to registration on a national database of study \& trial subjects to prevent over-volunteering (TOPS) * Willing to give consent for study investigators to contact the participants GP in the event of a significant abnormality being observed * Willing to show identification documents to confirm identity * Willing to give consent to biopsy(s) of suspected cutaneous leishmaniasis lesions Exclusion Criteria: * Receipt of any vaccine within 21 days of screening * Administration of immunoglobulins and/or any blood products within the three months preceding the planned study. * History of significant allergic disease/atopy (e.g. eczema, hay fever, asthma) or reactions; or a history of severe or multiple allergies to drugs or pharmaceutical agents, as judged by the clinical investigators * Any significant chronic skin condition as judged by the clinical investigators * Any history of confirmed Leishmaniasis infection * Any history of travel within the last 30 days to regions where Leishmania major-transmitting sand flies are endemic\*. * Any history of more than 30 continuous days stay in regions where Leishmania major-transmitting sand flies are endemic within the last 10 years\*. * Any history of severe local or general reaction to insect bites, defined as * Local: extensive, indurated redness and swelling involving most of the antero-lateral thigh or the major circumference of the arm, not resolving within 72 hours * General: fever ≥ 39.5°C, anaphylaxis, bronchospasm, laryngeal oedema, collapse, convulsions or encephalopathy within 48 hours * Any history of anaphylaxis * Females - current pregnancy, less than 12 weeks postpartum, lactating or willingness/intention to become pregnant during the study. * Any clinically significant abnormal finding on screening biochemistry or haematology blood tests as judged by study investigators * Total IgE (immunoglobulin E) levels \> 214 IU/ml * Any confirmed or suspected immunosuppressive or immunodeficient state, including HIV infection; asplenia; recurrent, severe infections and chronic (more than 14 days) immunosuppressant medication within the past 6 months * A diagnosis of diabetes type 1 or type 2 or significantly raised HbA1c (\>48mmol/mol) * Active Tuberculosis, leprosy, or malnutrition * Any significant chronic illness requiring hospital specialist input as judged by study investigators * Any significant psychiatric conditions as judged by general practitioner and/or study clinical team * Unlikely to comply with the study protocol * Participating in significant current or recent research (involving an investigational medicinal product or other significant intervention) within the past 3 months (as judged by study investigators) * Any other significant disease, disorder, finding or medical history, which, in the opinion of a medically qualified Clinical Investigator, may either put the participant at risk because of participation in the study, or may influence the result of the study, or the participant's ability to participate in the study