Human Intestinal Amino Acid Absorption and the Role of a Local RAS

University Children's Hospital Basel50 enrolled

Overview

This study is to find out what role a local intestinal RAS (renin angiotensin system) plays in the context of amino acid absorption in the human intestinal tract and how this RAS and thus the amino acid absorption is influenced by the RAS-active drugs (angiotensin II AT1 receptor blockers (sartans) or ACE inhibitors).

Study Type

OBSERVATIONAL

Enrollment

Conditions

Renin-Angiotensin Aldosterone System (RAS)

Interventions

biopsy of intestinal tissueOTHER

4 intestinal tissue biopsies taken from descending Duodenum, 2 intestinal tissue biopsies taken from Ileum, 2 intestinal tissue biopsies taken from ascending colon (additional to routine biopsies taken for gastrointestinal diagnostic)

blood drawOTHER

40 ml blood draw (in Heparin blood tubes) (additional to routine blood draw taken for gastrointestinal diagnostic)

urine collectionOTHER

Midstream Urine collection

Eligibility

Sex: ALLMin age: 18 YearsMax age: 80 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Body Mass Index (BMI): 18-35 kg/m2 * cohort 1: therapy with an angiotensin II AT1 antagonist or ACE Inhibitor * cohort 2: no therapy with an angiotensin II AT1 antagonist or ACE Inhibitor * Carrying out a diagnostic gastroscopy, colonoscopy or a combined gastroscopy / colonoscopy * Existence of written consent after detailed information about the study Exclusion Criteria: * Severe pathological changes in the gastrointestinal tract (e.g. sprue, stomach ulcers, malignancies); (reflux esophagitis and gastritis are not Exclusion criteria) * History of gastrointestinal tract surgery (except for appendectomy and inguinal hernia surgery) * History of malignancy * Severe acute and chronic organ diseases requiring treatment (e.g. kidney replacement therapy) * Patients with an increased risk of bleeding (e.g. oral anticoagulation, coagulation disorders) * Drug or alcohol abuse * Mental impairment limiting the ability to meet all study requirements

Locations (2)

Universitäts-Kinderspital beider Basel (UKBB)

Basel, Switzerland

UniversitätsSpital Zürich

Zurich, Switzerland

Outcomes

Primary Outcomes

messenger ribonucleic acid (mRNA) quantification in intestinal tissue biopsy

mRNA quantification in intestinal tissue biopsy: mRNA content coding for regulatory RAS protein is measured relative to the mRNA content, which codes for the structural protein villin.

Time frame: single time-point at baseline

amino acid concentration in blood sample

amino acid concentration in blood sample by High Performance Liquid Chromatography (HPLC)

Time frame: single time-point at baseline

amino acid concentration in urine sample

amino acid concentration in urine sample by HPLC (High Performance Liquid Chromatography)

Time frame: single time-point at baseline

Data from ClinicalTrials.gov

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