Skin graft is a commonly used method in wound repair. The long-term outcome of skin graft is unsatisfied because of the complications of contraction, pigmentation and stiffness. In our preclinical researches, we found that adipose stem cell can improve the texture of grafted skin and reduce contraction. In this trial, we will evaluate the efficiency and safety of autologous adipose stem cells in improving skin graft outcomes.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
DOUBLE
Enrollment
75
Abdominal or buttock fat will be aspirated under local anesthesia or general anesthesia by conventional methods (0.5 ml fat was aspirated per 1 cm2 of skin graft area). The matrix component rich in adipose stem cells will be obtained by chopping, washing and centrifugation. The intervention treatment will be performed after the skin graft sutured. The stem cell components will be injected underneath the skin graft (0.05 ml/cm2).
saline will be injected underneath skin graft (0.05 ml/cm2).
Skin elasticity
Skin elasticity analyzed by Cutometer. The R2 value is used to evaluated the skin elasticity.
Time frame: 6 months
Skin elasticity
Skin elasticity analyzed by Cutometer. The R2 value is used to evaluated the skin elasticity.
Time frame: 3 months
Skin elasticity
Skin elasticity analyzed by Cutometer. The R2 value is used to evaluated the skin elasticity.
Time frame: 12 months
Skin color
Skin color analyzed by CK Colorimeter CL400. The iTA value is used to evaluate the color of skin graft.
Time frame: 3 months
Skin color
Skin color analyzed by CK Colorimeter CL400. The iTA value is used to evaluate the color of skin graft.
Time frame: 6 months
Skin color
Skin color analyzed by CK Colorimeter CL400. The iTA value is used to evaluate the color of skin graft.
Time frame: 12 months
POSAS questionnaire of patient
POSAS questionnaire of patient. Five questions of pain, itch, thickness, color, stiffness, irregularity. Each question ranks 0, 1, 2. Total score is 0 to 10.
Time frame: 3 months
POSAS questionnaire of patient
POSAS questionnaire of patient. Five questions of pain, itch, thickness, color, stiffness, irregularity. Each question ranks 0, 1, 2. Total score is 0 to 10.
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Time frame: 6 months
POSAS questionnaire of patient
POSAS questionnaire of patient. Five questions of pain, itch, thickness, color, stiffness, irregularity. Each question ranks 0, 1, 2. Total score is 0 to 10.
Time frame: 12 months
POSAS questionnaire of investigator
POSAS questionnaire of investigator. Six questions of vascularity, pigmentation, texture, thickness, pliability, surface area. Each question ranks 0, 1, 2. Total score is 0 to 12.
Time frame: 3 months
POSAS questionnaire of investigator
POSAS questionnaire of investigator. Six questions of vascularity, pigmentation, texture, thickness, pliability, surface area. Each question ranks 0, 1, 2. Total score is 0 to 12.
Time frame: 6 months
POSAS questionnaire of investigator
POSAS questionnaire of investigator. Six questions of vascularity, pigmentation, texture, thickness, pliability, surface area. Each question ranks 0, 1, 2. Total score is 0 to 12.
Time frame: 12 months