Effect of Total Sleep Deprivation on Vascular Function

Inclusion criteria: * Males and females * 20 to 30 and 50 to 60 years of age * Females will be eumenorrheic at enrollment or postmenopausal for at least 1 year * No major clinical disease (e.g., diabetes, cardiovascular, liver or chronic kidney disease) to minimize confounding of vascular measures * Participants will be sedentary (≤3x per week of ≤30 minutes of aerobic exercise/session) or trained (≥5x per week of moderate/vigorous aerobic exercise training) Exclusion criteria: * Age \<20 or 31 to 49 or \>60 years * Body mass index ≥30 kg/m\^2 because obesity may affect vascular function * Use of medication that may affect vascular measures * Hormone replacement therapy or hormonal contraceptives within past year * Use of tobacco products (chewing tobacco, traditional or e-cigarettes) because they may influence vascular function * Being perimenopausal, pregnant or lactating because may influence vascular function * Being a shift worker because habitual sleep deprivation and altered circadian rhythm may influence vascular function. * Not having an "intermediate" chronotype based on the Morningness-Eveningness Questionnaire (MEQ; score \<31 or \>69) because this would influence the effect of overnight sleep deprivation on vascular function. * Sleep complaints based on the Pittsburgh Sleep Quality Index (PSQI; score \>5) because this would influence the effect of overnight sleep deprivation on vascular function.