To investigate whether telomere length (TL) of the first Polar body (PB) correlates with TL in Cumulus cells (CC)
With the present study we want to analyze, as a primary objective, whether telomere length (TL) in the first Polar Body (PB) correlates with TL in the corresponding Cumulus cells (CC). As a secondary objective, a possible correlation between TL in PBs and blastocyst TL and ploidy will be evaluated. To our best knowledge, there are no studies evaluating TL in CC and TL in PBs of the corresponding oocyte. With the present prospective study, we sought to investigate whether there is a correlation between CC-TL and PB-TL. If a correlation exists, CC-TL assessment would serve as a valuable non-invasive technique to gain information about oocyte competence.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
DIAGNOSTIC
Masking
NONE
Enrollment
10
Since PBs are by-products of the meiotic division of the oocyte and are not required for fertilization and subsequent embryo development, they can be removed to assess exclusively maternal chromosomal information without harming the embryo integrity. As previously described, PB biopsy does not impact the morphokinetic parameters of the embryo development and can be safely applied without the risk of impairing the reproductive potential of the embryo.
preimplantation genetic screening for aneuploidies
ART Fertility Clinics
Abu Dhabi, United Arab Emirates
RECRUITINGtelomere length (TL) of the first Polar body (PB) and TL in Cumulus cells (CC)
Correlation between telomere length (TL) of the first Polar body (PB) and TL in Cumulus cells (CC)
Time frame: 8 weeks
To correlate TL between both polar bodies
If the MII fertilize after performing ICSI, polar body biopsy for the second PB will be performed
Time frame: 8 weeks
To correlate TL in male WBC and TL in sperm
TL will be assess in leukocytes (male´s blood sample) and in the sperm (fresh ejaculate)
Time frame: 8 weeks
To correlate TL in female WBC and TL in CC
TL will be assess in leukocytes (female´s blood sample) and in the cumulus cells (CC) from the retrieved oocytes.
Time frame: 8 weeks
To evaluate a possible correlation between TL-CC and TL-TE
Correlation between TL-CC and TL in trophectoderm biopsy performed in the developed blastocysts
Time frame: 8 weeks
To evaluate a possible correlation between TL-CC and ploidy TE
Correlation between TL-CC and PGT-A results in trophectoderm biopsy performed in the developed blastocysts
Time frame: 8 weeks
To evaluate telomerase activity (TA) in CC and a possible correlation with CC-TL
Telomerase is a ribonucleoprotein complex responsible for de novo telomere synthesis and addition of telomeric repeats to existing telomeres
Time frame: 8 weeks
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To evaluate FSH/LH gene expression in CC
During the follicular phase LH whose receptors (LHR) are expressed in granulosa cells under the influence of FSH, plays an important role in the regulation of ovarian function and is essential to promote the growth of the dominant follicle, final oocyte maturation as well as ovulation induction
Time frame: 8 weeks
To evaluate a possible correlation between ploidy in PBs and ploidy in TE biopsy
PGT-A in PB and PGT-A in TE biopsy
Time frame: 8 weeks
PB biopsy and embryo development
To validate that PB biopsy does not impair embryo development compared to their sibling embryos with no PB biopsied
Time frame: 8 weeks
To evaluate TL in sperm + TL-PB and TL in TE
TL in sperm + TL-PB and TL in the resulting biopsied
Time frame: 8 weeks