Periodontitis is an inflammatory disease characterized by a dysbiotic microbiome which can lead to bone destruction and tooth loss. Several studies had been reported the association to periodontal disease with systemic conditions and this relation suggests and axis that links oral and gut microbiome. In order to clarify the impact of periodontal condition on gut microbiome, we aim to evaluate the clinical, immunological parameters and the microbiological condition by sequencing of subgingival biofilm and stool samples both before and after non-surgical periodontal treatment with and without antibiotics as adjunct.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
TRIPLE
Enrollment
30
Periodontal debridement
Administration of Amoxicillin 500mg prescribed on the day of treatment tid for 7 days.
Administration of Metronidazole 400mg prescribed on the day of treatment tid for 7 days.
Administration of Placebo on the day of treatment tid for 7 days.
Administration of Placebo on the day of treatment tid for 7 days.
University of Campinas, UNICAMP
Piracicaba, São Paulo, Brazil
RECRUITINGChange in the oral-gut axis microbiome at 3 months
Concentration of bacteria in the subgingival biofilm and stool samples
Time frame: Baseline and 90 days
Change of biofilm gene expression on oral microbiome at 3 months
RNA-seq of 3 samples FMUD+AM group and validation by quantitative polymerase chain reaction
Time frame: Baseline and 90 days
Change of the baseline inflammatory markers levels in gingival crevicular fluid (pg/uL) and stool samples
Concentration of cytokines and biomarkers in gingival crevicular fluid and in stool samples
Time frame: Baseline and 90 days
Change of the baseline relative clinical attachment level at 3 months
Distance from the bottom of the pocket to the stent margin.
Time frame: Baseline, 30 days and 90 days
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