septic shock is the first cause of mortality in intensive care unit. Innate immunity is the body's first line of defense against pathogens
since their discovery, inflammasomes have an important role during inflammatory response following an aggression. There are intracytoplasmic multiprotein complex activated by cellular stress or infections and is responsible for the release of pro-inflammatory cytokines, including IL-1β. One study focused on the role of the NLRP3 inflammasome in monocyte during sepsis has shown its early alteration that is correlated with mortality. Most studies have analysed inflammasomes in nucleated cells, nevertheless, little is known about inflammasomes in platelets. The aim of the study is to analyze the activation of NLRP3 inflammasome in platelets and leukocytes during sepsis, from patients hospitalized in intensive care unit compare to a control group, until its resolution to better understand the pathophysiology of sepsis
Study Type
OBSERVATIONAL
Enrollment
11
Blood collection (at admission, two days, one week and at discharge) to analyze the activation of NLRP3 inflammasome in platelets and leukocytes
University Hospital of Toulouse
Toulouse, France, France
Activation kinetics of the NLRP3 inflammasome during sepsis until its resolution
Activation kinetics of the NLRP3 inflammasome from day 0 to patient discharge measured by the area under curve
Time frame: From day 0 to the hospital discharge, an average of 1 month
Rate of inflammasome NLRP3 activation
Rate of inflammasome NLRP3 activation (measured in blood samples)
Time frame: Day 0
Rate of inflammasome NLRP3 activation
Rate of inflammasome NLRP3 activation (measured in blood samples)
Time frame: Day 2
Rate of inflammasome NLRP3 activation
Rate of inflammasome NLRP3 activation (measured in blood samples)
Time frame: Day 7
Rate of inflammasome NLRP3 activation
Rate of inflammasome NLRP3 activation (measured in blood samples)
Time frame: Day of hospital discharge, an average of 1 month
Severity score measured by Sequential Organ Failure Assessment (SOFA) score
Severity score measured by Sequential Organ Failure Assessment (SOFA) score, from 0 to 4; higher score mean worse outcome
Time frame: Day 0
Severity score measured by Sequential Organ Failure Assessment (SOFA) score
Severity score measured by Sequential Organ Failure Assessment (SOFA) score, from 0 to 4; higher score mean worse outcome
Time frame: Day 2
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Severity score measured by Sequential Organ Failure Assessment (SOFA) score
Severity score measured by Sequential Organ Failure Assessment (SOFA) score, from 0 to 4; higher score mean worse outcome
Time frame: Day 7
Severity score measured by Sequential Organ Failure Assessment (SOFA) score
Severity score measured by Sequential Organ Failure Assessment (SOFA) score, from 0 to 4; higher score mean worse outcome
Time frame: Day of hospital discharge, an average of 1 month
Severity score measured by Simplified Gravity Index 2
Severity score measured by Simplified Gravity Index 2, score from 0 to 163, higher score mean worse outcome
Time frame: Day 0
Severity score measured by Simplified Gravity Index 2
Severity score measured by Simplified Gravity Index 2, score from 0 to 163, higher score mean worse outcome
Time frame: Day 2
Severity score measured by Simplified Gravity Index 2
Severity score measured by Simplified Gravity Index 2, score from 0 to 163, higher score mean worse outcome
Time frame: Day 7
Severity score measured by Simplified Gravity Index 2
Severity score measured by Simplified Gravity Index 2, score from 0 to 163, higher score mean worse outcome
Time frame: Day of hospital discharge, an average of 1 month