Fecal Microbiota Transplantation in Postoperative Crohn's Disease

Safety of FMT using FinFMT-Questionnaire

Unvalidated survey including 20 questions.

Time frame: 3 months, 12 months, 5 years

Clinical activity of Crohn's disease using Harwey-Bradshaw Index

Scores of less that 5 indicate that the patient's Crohn's disease is in remission while scores higher than 16 indicate severe disease activity. Median scores will be compared between intervention group and placebo group.

Time frame: 6 months, 12 months, 5 years

Histologic activity of Crohn's Disease using modified Global Histological Activity Score

The GHAS consists of eight items assessing acute and chronic inflammatory changes, epithelial damage and the extent of inflammation (i.e. the proportion of biopsy specimens affected). Each of the eight items is scored, with the totals subsequently added together. Maximum number 14 indicates histological activity. The median GHAS score will be compared between intervention group and placebo group.

Time frame: 1 years, 5 years

Change of microbiota in stool samples and in intestinal biopsies

The relative abundances of taxonomic units in cases versus controls and different time points will be compared at the level of phylum, class, genus, species and OTU. Alpha diversity measures will be determined from the original unfiltered dataset by counting observed taxa (OTU richness) and by Chao1, ACE, Shannon, Simpson and Fisher indices; diversity will be compared between time points.cases and controls. Beta diversity will be assessed by examining the results of principle component analysis using multiple distance methods, if appreciable difference between cases and controls or time points will be noted, formal testing of clustering will be performed. To simplify complex bacteriome profiles, we will also sort each sample into enterotypes based on the leading components of their bacteriomes; the enterotypes will be tested as a predictor of the case-control or disease status of cases.