Effect of Dental Pulp Stem Cells and L-PRF After Impacted Third Molar Extraction

Baskent University13 enrolled

Overview

The purpose of this study is to compare the clinical and radiographic effectiveness of Leukocyte-Platelet Rich Fibrin (L-PRF) and L-PRF combined with dental pulp stem cell (DPSC) application to the extraction socket of mandibular third molars.

Patients aged between 18 and 30 years, seeking for bilateral impacted LM3 extraction surgeries and meeting eligibility criteria were included to the study. At baseline right and left impacted third molars (LM3s) were randomly assigned to one of the treatment group (L-PRF and L-PRF + DPSC). Baseline and 6th month clinical and radiographic measurements were compared.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

PREVENTION

Masking

DOUBLE

Enrollment

Conditions

Stem Cell Third Molar Periodontal Pocket

Interventions

L-PRF + DPSCPROCEDURE

After surgical removal of impacted mandibular third molars, extraction sockets were filled with L-PRF membranes + DPSCs.

L-PRFPROCEDURE

After surgical removal of impacted contralateral mandibular third molars, each extraction sockets were only filled with L-PRF membranes.

Eligibility

Sex: ALLMin age: 18 YearsMax age: 30 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Patients aged between 18 and 30 years * Bilateral impacted LM3 extraction surgeries requirement Exclusion Criteria: * Having clinical signs or symptoms of abscess or cellulitis formation * Having a history of radiotherapy * Having any systemic diseases interfering with wound healing and/or smokers * Patients who had been diagnosed as periodontitis * Patients who had LM2s with caries or restorations that have an unidentifiable cementoenamel junction

Locations (1)

Baskent University

Ankara, Turkey (Türkiye)

Outcomes

Primary Outcomes

Probing pocket Depth (PPD)

Probing pocket Depth (PPD) is the distance between gingival margin and bottom of the periodontal pocket in mm.

Time frame: 6 months

Clinical attachment level (CAL)

Clinical attachment level (CAL) is the distance between cemento-enamel junction and bottom of the periodontal pocket in mm.

Time frame: 6 months

Secondary Outcomes

Radiographic vertical distance

The cementoenamel junction (CEJ) and the most coronal point of the alveolar bone were identified (AC) on the mesial and distal of lower second molars on digital panoramic radiographs. The vertical ruler on each digital image was used to calibrate the image and to convert measurements from pixels to millimeters with ImageJ. The vertical distance (VD) between cemento-enamel junction and alveolar crest of the lower second molar was than measured with "straight line" tool of the software. In order to calculate the real distance; ertical distance was divided to the magnification factor for panoramic image (1.2).

Time frame: 6 months

Relative bone density (rBD)

For every extraction socket, a region of interest (ROI) is created for the socket region and one for the surrounding bone using the "Freehand Selection" tool of ImageJ software on panoramic radiographs. Mean grey values were recorded for each group and relative bone density (rBD) was calculated.

Time frame: 6 months

Post-operative pain as assessed by visual analogue scale (VAS)

Self-reported pain measured in a 100 mm visual analogue scale (VAS) in which (0) denoted "no pain" and 100 meant "severe pain" was recorded at the end of the surgery and 7 days after extraction.

Time frame: 7 days

Data from ClinicalTrials.gov

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