Tissue plasminogen activator (tPA) is the FDA approved thrombolytic drug for patients with acute ischemic stroke. While transcatheter endovascular therapy is widely accepted as an effective method for targeted blood vessels via a catheter to elicit ischemic necrosis. To improve targeting accuracy administrated tPA and avoiding collateral tissue damage, we developed a magnetic nanorobotic system to active system to active intravenous delivery of tPA for targeted thrombus removal. Here human placenta will be used as an ex vivo model for human cerebral vascular system in our ex vivo thrombolysis study. Our magnetic nanorobotic system encapsulated with tPA overcomes the limitations of passive thrombolytic therapy with tPA alone and will be a promising approach for the treatment of ischemic stroke and other thrombotic diseases
Study Type
OBSERVATIONAL
Enrollment
100
Advanced Nanomaterials & Microrobotics Laboratory, Room 202, William M.W Mong Engineering Building, CUHK and Room 508, Li Ka Shing Medical Sciences Building, Prince of Wales Hospital
Shatin, New Territories, Hong Kong
ACTIVE_NOT_RECRUITINGDepartment of Obstetrics and Gynaecology, Prince of Wales Hospital
Shatin, New Territories, Hong Kong
RECRUITINGThrombolysis performance
Size of thrombus by angiography, mm
Time frame: 1 -2 hours
Time for recanalization
Time for recanalization of occluded vessels, mins
Time frame: 1 -2 hours
Damage to vessels
Histological damage evaluation. The vessels are sectioned and stained with H\&E stain
Time frame: 1 -2 hours
Stability of magnetic nanorobots
Controllability of the magnetic nanorobots. The loss in mass of nanorobots after magetic navigation in blood vessels is measured.
Time frame: 1 - 2 hours
Dosage requirement
Dosage requirement of nanorobots and thus t-PA, mg
Time frame: 1 - 2 hours
Distribution of administrated nanorobots
The distribution of administrated nanorobots. The position of nanorobots is tracked by MRI.
Time frame: 1 - 2 hours
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