Evaluating the Bacterial and Fungal Microbiome in Patients With Calcium Oxalate Urolithiasis and Renal Cell Carcinoma

University of Miami90 enrolled

Overview

The purpose of the study is to identify bacterial and fungal microbiome associated with calcium oxalate (CO) urolithiasis and renal cell carcinoma (RCC).

Study Type

OBSERVATIONAL

Enrollment

Conditions

Calcium Oxalate Urolithiasis Renal Cell Carcinoma

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Adults that are able to consent (≥ 18 years of age) * No history of asymptomatic bacteriuria or Urinary Tract Infection within the past 1 month * No history of coagulopathy and not on anticoagulant or antiplatelet medication at the time of surgery. * For patients with urolithiasis, must not have a history of RCC * For patients with RCC, must not have a history of urolithiasis * Controls must have no history of stones, RCC, or urinary tract infection (within 1 month). * For all participants, no antimicrobial treatment within the past month Exclusion Criteria: * Adults unable to consent * For patients with urolithiasis, technical difficulty in taking renal papillae biopsy. * History of urinary tract infection within the past 1 month * History of coagulopathy and on anticoagulants or antiplatelets that could not be stopped perioperatively. * Patients with nephrolithiasis and renal tumors with positive preoperative urine cultures- suggesting asymptomatic bacteriuria. * For patients with urolithiasis, a history of RCC * For patients with RCC, a history of urolithiasis * Controls who have a history of stones, RCC, or urinary tract infection. * For all participants, antimicrobial treatment within the past month.

Locations (1)

University of Miami

Miami, Florida, United States

Outcomes

Primary Outcomes

Microbial community structure in patients with CO urolithiasis

Alpha and beta diversity, principal coordinate analysis, and phylogenetic trees of the bacterial and fungal microbial community from mid-stream voided urine sample, urine sample collected from the bladder, kidney stone fragment, and a renal papillae biopsy specimen.

Time frame: Day 1

Microbial community structure in patients with RCC

Alpha and beta diversity, principal coordinate analysis, and phylogenetic trees of the bacterial and fungal microbial community from catheterized urine specimen from the bladder, a biopsy of the tumor, and a biopsy of normal-appearing renal papillae specimen.

Time frame: Day 1

Microbial community structure in healthy participants

Alpha and beta diversity, principal coordinate analysis, and phylogenetic trees of the bacterial and fungal microbial community from clean-catch urine specimen.

Time frame: Day 1

Data from ClinicalTrials.gov

This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.