The aim of this study is to investigate urokinase Plasminogen Activator Receptor (uPAR) microexpression in gastroesophageal cancer (adenocarcinomas) both qualitatively and semi-quantitatively and to evaluate if it offers a possibility for future imagining purposes.
* Sections of the tumor tissue will be HE stained. * Sections of the tumor tissue will be stained with an uPAR antibody for immunohistochemistry. * Immunohistochemical staining against cytokeratin which colors cancer cells will be made to assist the observer in the evaluation of uPAR microexpression pattern. * A semiqualitative scale corresponding to the level of uPAR expression will be made. * Quantitative polymerase chain reaction will be used to validate the immunohistochemistry uPAR expression pattern. * An electronic scoring system (digital pathology) will be trained to access the expression of uPAR in both tumor- and normal tissue.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
OTHER
Masking
NONE
Enrollment
100
Resected esophageal cancer will be qualitatively and semi-quantitatively analyzed for uPAR expression
Rigshospitalet
Copenhagen, Capital Region, Denmark
uPAR microexpression
Our primary objective is to investigate uPAR microexpression in gastroesophageal adenocarcinomas both qualitatively and semi-quantitatively. This will be done by a pathology analysis of gastroesophageal cancer resections.
Time frame: Up to 6 months
Tumor-to-background ratio
A pathology staining will determine the uPAR in the tumor compared with the healthy cells.
Time frame: Up to 6 months
uPAR in patient groups
Baseline statistical analysis of patient groups and their expression of uPAR in their resected tumor tissues will be made.
Time frame: Up to 6 months
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.