Post-stroke cognitive impairment(PSCI) is one of the most important factors causing disabilities after stroke. Recent study found that gut microbiota plays a key role in neurological diseases. Two recent small sample studies reported gut dysbiosis in PSCI patients. In order to further verify the relationship between PSCI and gut microbiota and the predictive value of gut microbiota and serum markers for cognitive impairment and poor prognosis after ischemic stroke. The study intended to collect stool specimens of patients with acute ischemic stroke and assess their cognitive psychological state, and to establish a prospective multi-center follow-up cohort to explore the correlation between the dynamic changes of intestinal flora in patients with stroke and PSCI and poor prognosis of stroke.
Study Type
OBSERVATIONAL
Enrollment
600
Department of Neurology, NanFang Hospital, Southern Medical University
Guanzhou, Guangdong, China
RECRUITINGMini-Mental State Examination
A cognitive function screening test ranged 0-30, higher scores mean better cognitive function
Time frame: 7 days after admission
Mini-Mental State Examination
A cognitive function screening test ranged 0-30, higher scores mean better cognitive function
Time frame: 3 months after discharge
Mini-Mental State Examination
A cognitive function screening test ranged 0-30, higher scores mean better cognitive function
Time frame: 6 months after discharge
Montreal Cognitive Assessment
A cognitive function screening test ranged 0-30, higher scores mean better cognitive function
Time frame: 7 days after admission
Montreal Cognitive Assessment
A cognitive function screening test ranged 0-30, higher scores mean better cognitive function
Time frame: 3 months after discharge
Montreal Cognitive Assessment
A cognitive function screening test ranged 0-30, higher scores mean better cognitive function
Time frame: 6 months after discharge
Gut microbiota
Results of fecal bacteria by 16s RNA sequencing
Time frame: 2 days after admission
Gut microbiota
Results of fecal bacteria by 16s RNA sequencing
Time frame: 3 months after discharge
Modified Rankin Scale(mRS)
A neurological function score scale ranged 0-6, higher scores mean worse neurological outcome
Time frame: 7 days after admission
Modified Rankin Scale(mRS)
A neurological function score scale ranged 0-6, higher scores mean worse neurological outcome
Time frame: 3 months after discharge
Modified Rankin Scale(mRS)
A neurological function score scale ranged 0-6, higher scores mean worse neurological outcome
Time frame: 6 months after discharge
Modified Rankin Scale(mRS)
A neurological function score scale ranged 0-6, higher scores mean worse neurological outcome
Time frame: 12 months after discharge
National Institute of Health stroke scale(NIHSS)
Neurological function score scale, ranged 0-42, higher scores mean more severe neurological deficit
Time frame: Day 1 of admission
National Institute of Health stroke scale(NIHSS)
Neurological function score scale, ranged 0-42, higher scores mean more severe neurological deficit
Time frame: Day 7 of admission
National Institute of Health stroke scale(NIHSS)
Neurological function score scale, ranged 0-42, higher scores mean more severe neurological deficit
Time frame: 3 months after discharge
National Institute of Health stroke scale(NIHSS)
Neurological function score scale, ranged 0-42, higher scores mean more severe neurological deficit
Time frame: 6 months after discharge
Short chain fatty acids
A metabolites of gut microbiota from stool, detected by gas chromatography-mass spectrometry (GC-MS) combined technique
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Time frame: 2 days after admission
Short chain fatty acids
A metabolites of gut microbiota from stool, detected by gas chromatography-mass spectrometry (GC-MS) combined technique
Time frame: 3 months after discharge
Trimethylamine-N-Oxide
A metabolites of gut microbiota in plasm, quantified by stable isotope dilution liquid chromatography tandem mass spectrometry
Time frame: 2 days after admission
Trimethylamine-N-Oxide
A metabolites of gut microbiota in plasm, quantified by stable isotope dilution liquid chromatography tandem mass spectrometry
Time frame: 3 months after discharge
Untargeted Metabolomics
Untargeted metabolomics refers to using gas chromatography-mass spectrometry (GC-MS) combined technique, without bias detection of all small molecule metabolites in plasma (mainly the relative molecular weight of 1000 Da endogenous small molecule compounds) levels.
Time frame: 2 days after admission
Untargeted Metabolomics
Untargeted metabolomics refers to using gas chromatography-mass spectrometry (GC-MS) combined technique, without bias detection of all small molecule metabolites in plasma (mainly the relative molecular weight of 1000 Da endogenous small molecule compounds) levels.
Time frame: 3 months after discharge