The investigators will study nasopharunx microbiota composition, RNA sequences and in vitro cytokines production by peripheral blood cells of COVID-19 patients (both mild-moderate and severe cases) (comparing with convalescent patients and healthy controls).
The investigators will compare study parameters (basal) between mild-moderate and severe COVID-19 patients and with other study groups (i.e. convalescent and healthy controls). Nasopharunx microbiota dysbiosis, in vitro cytokines production profile, and peripheral blood flocytometric results of the four study groups will be compared. In vitro cytokines production will be studied and compared in Nill (No antigens) and Mitojen (Cocktail of antigens) pre-prepared test tubes.
Study Type
OBSERVATIONAL
Enrollment
100
The study parameters will be obtained at the time of recuirement (Basal)
Bakirkoy Dr.sadi Konuk Training and Research Hospital
Istanbul, Turkey (Türkiye)
RECRUITINGBakirkoy Dr.sadi Konuk Training and Research Hospital
Istanbul, Turkey (Türkiye)
RECRUITINGDetermination of peripheral blood RNA microarray assay
Four study groups, microarray Analysis .
Time frame: First day
Determination of nasopharunx Microbiota analysis
Four study groups, metagenomic analysis, to determine abundance and decrease of some species.
Time frame: 1st day
Determination of in vitro cytokines production
IL-2, IL-7, IL-10, granulocyte-colony stimulating factor, interferon-p inducible protein 10, monocyte chemo-attracting protein 1, macrophage inflammatory protein 1-α and tumor necrosis factor-α in Nill (no angijen) and Mitojen (Cocktails of antigens)tubes (between 4 study groups)
Time frame: First day
Determination of peripheral blood cytometry
Four study groups, peripheral blood cytometry; CD3, CD4, CD8, and CD45 rates.
Time frame: First day
Relation between study parameters
determining the realtion between microbiotme dysbiosis, in vitro cytokines production and peripheral leymphocytes (CD3,CD4, CD8, and CD45) rates and the severity of COVID-19( if any)
Time frame: First day parameters
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.