Lactate may have anti-lipolytic effects when plasma concentrations of lactate reach levels similar to those seen during high intensity exercise. This study aims to investigate how lactate concentrations similar to those achieved during high intensity exercise affects lipolysis in humans. In addition to this, to investigate how increased lactate concentrations affects glucose- and amino acid metabolism. 8 healthy males will be included. Study participants will undergo two separate investigation days that will be identical except for the interventions: 1. Intravenous sodium D/L-lactate 2. Intravenous sodium chloride. The study consists of a 3-hour basal period followed by a 3-hour hyperinsulinemic euglycemic clamp. During the study we will: * Estimate insulin sensitivity during the hyperinsulinemic euglycemic clamp (M value) * Use tracer kinetics to estimate lipid-, glucose and amino acid metabolism using \[9,10-3H\]-palmitate, \[3-3H\]-glucose, \[15N\]-phenylalanine, \[15N\]-tyrosine, \[2H4\]-tyrosine and \[13C\]-Urea. * Do muscle- and adipose tissue biopsies for analyses of signaling pathways involved in regulation of lipid-, glucose and amino acid metabolism. * Do blood samples of relevant hormones, metabolites and cytokines. * Use indirect calorimetry to estimate study participants' resting energy expenditure and respiratory quotient during the basal period. * Estimate cardiac ejection fraction by echocardiography and measure blood pressure during both the basal- and clamp period.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
SINGLE
Enrollment
8
intravenous
Intravenous
Medical/Steno Aarhus research laboratory
Aarhus, Central Region Denmark, Denmark
Aarhus University Hospital
Aarhus N, Denmark
Acute Metabolic effects of Na-lactate compared with NaCl on lipolysis
Change in \[9,10-3H\]-palmitate tracer flux
Time frame: 2 hours (1 hour basal period, 1 hour clamp)
Acute Metabolic effects of Na-lactate compared with NaCl on glucose metabolism
Change in glucose metabolism quantified with \[3-3H\]-glucose tracer
Time frame: 6 hours (3 hours basal period, 3 hours clamp)
Insulin sensitivity
Hyperinsulinaemic euglycaemic clamp with 0.6mU/kg/min insulin and 20% glucose to calculate M value. Difference between interventions.
Time frame: 3 hours.
Acute metabolic effects of Na-lactate compared with NaCl on aminoacid metabolism
Change in aminoacid metabolism quantified with \[15N\]-phenylalanine, \[15N\]tyrosine, \[2H4\]tyrosine and \[13C\]-Urea tracers.
Time frame: 6 hours
Indirect calorimetry during clamp
Change in estimation of resting energy expenditure during clamp. Difference between interventions.
Time frame: 20 minutes
Indirect calorimetry during basal conditions
Change in estimation of respiratory quotient during basal period. Difference between interventions.
Time frame: 20 minutes
Echocardiography
Change in cardiac ejection fraction during basal period. Difference between interventions.
Time frame: 15 minutes
Echocardiography
Change in cardiac ejection fraction during clamp period. Difference between interventions.
Time frame: 15 minutes
blood pressure
blood pressure (systolic and diastolic) during basal- and clamp
Time frame: 2 x 10 min
Blood samples - Insulin
Insulin concentrations. Difference between interventions.
Time frame: 6 hours.
Blood samples - c-peptid
c-peptid concentrations. Difference between interventions.
Time frame: 6 hours.
Blood samples - glucagon
glucagon concentrations. Difference between interventions.
Time frame: 6 hours.
Blood samples - cortisol
cortisol concentrations. Difference between interventions.
Time frame: 6 hours.
Blood samples - Ghrelin
Ghrelin concentrations. Difference between interventions.
Time frame: 6 hours.
Blood samples - GDF-15
GDF-15 concentrations. Difference between interventions.
Time frame: 6 hours.
Blood samples - free fatte acids
Free fatte acids concentrations. Difference between interventions.
Time frame: 6 hours.
Blood samples - glucose
glucose concentrations. Difference between interventions.
Time frame: 6 hours.
Blood samples - lactate
lactate concentrations. Difference between interventions.
Time frame: 6 hours.
Changes in signaling in muscle tissue between interventions
Western blot examinations of muscle tissue biopsies
Time frame: After 3 and 5 hours of intervention
Changes in signaling in adipose tissue between interventions
Western blot examinations of adipose tissue biopsies
Time frame: After 3 and 5 hours of intervention
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