Lemon Verbena Extract Oxidative Stress and Muscle Damage

inflammation of IL-6 level

To assess inflammation indicators, volunteers fasted for at least 8 hours before performing muscle exhaustion exercises. At designated time points in the recovery period, including baseline (0) and 3h, 24h, and 48 after exercise, blood samples were collected with brachial venous catheters. Assess serum IL-6 levels (pg/ml) to monitor physiological fitness. Used commercial kit human IL-6 immunoassay (R\&D system, Minnesota, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis

Time frame: 10 days

Clinical Biochemistry of CK level

To assess muscle damage, volunteers fasted for at least 8 hours before performing muscle exhaustion exercises. At designated time points in the recovery period, including baseline (0) and 3h, 24h, and 48 after exercise, blood samples were collected with brachial venous catheters. Serum CK (U/L), were assessed for monitoring physiological adaptation. All biochemical indices were assessed using an autoanalyzer (Hitachi 7060, Tokyo, Japan).

Time frame: 10 days

Stress hormones 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) in Urine

Subjects' urine was collected 24 hours after the exhaustive exercise for analysis. Used commercial kit human 8-OHdG (Cayman, Mich, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis

Time frame: 10 days

Assessment of muscle stiffness

3 h, 24h, 48h after exhausting exercise, use Myoton machine to evaluate muscle stiffness (N/m) (Myoton, Tallinn, Estonia)

Time frame: 10 days

Visual Analogue Scale (VAS) evaluation

3 h, 24h, 48h after exhausting exercise, use VAS questionnaire to record muscle pain. the minimum is 0 and maximum is 10, and whether higher scores mean more pain

Time frame: 10 days