CIDP, a rare disorder affecting young adults, causes gradual weakness of the limbs, areflexia and impaired sensory function. New CIDP phenotypes without antibodies but with modified cell profiles have been described. Treatments include corticotherapy, IVIg and plasmapheresis but the latter's action mechanisms remain unclear. Plasmapheresis supposedly removes toxic agents like antibodies from plasma but it is uncertain whether it has an immune-modulating effect. Also, the refining mechanisms of the two main plasmapheresis techniques - single plasma exchange and double filtration plasmapheresis (DFPP) - are different and unclear. This study aims to compare the evolution of peripheral lymphocyte profiles in patients with CIDP according to their treatment (single centrifugation plasmapheresis or DFPP) to better grasp the action mechanisms of both techniques.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
SINGLE
Enrollment
8
Blood sampling before and after each type of cycle, Single Plasma Exchange or Double Filtration Plasmapheresis
Olivier MORANNE
Nîmes, France
Cycle 1, Group A : TH1 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Time 0
Cycle 1, Group A : TH1 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 1, Group A : TH1 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 7 -10
Cycle 1, Group A : TH17 cells BEFORE first single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Time 0
Cycle 1, Group A : TH17 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 1, Group A : TH17 cells 7 - 10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.
Time frame: Day 7 - 10
Cycle 1, Group A : T-reg cells BEFORE first single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 1, Group A : T-reg cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 1, Group A : T-reg cells 7 - 10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 7 - 10
Cycle 1, Group B : TH1 cells BEFORE first double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 1, Group B : TH1 cells AFTER the first double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 1, Group B : TH1 cells 7-10 days AFTER the first double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 7 - 10
Cycle 1, Group B : TH17 cells BEFORE the first double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day0, Hour 0
Cycle 1, Group B : TH17 cells AFTER the first double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 1, Group B : T17 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 7-10
Cycle 1, Group B : T-reg cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 1, Group B : T-reg cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 1, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 7 - 10
Cycle 2, Group A: TH1 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 2, Group A: TH1 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 2, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %.
Time frame: Day 7 - 10
Cycle 2, Group A: TH17 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 2, Group A: TH17 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 2, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 7-10
Cycle 2, Group A: T-reg cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 2, Group A: T-reg cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 2, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 7-10
Cycle 2, Group B: TH1 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 2, Group B: TH1 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 2, Group B: TH1 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.
Time frame: Day 7-10
Cycle 2, Group B: T17 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T17 cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 2, Group B: TH17 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T17 cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 2, Group B: TH17 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time frame: Day 7-10
Cycle 2, Group B: T-reg cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 2, Group B: T-reg cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 2, Group B: T-reg cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 7-10
Cycle 3, Group A: TH1 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 1, Hour 1
Cycle 3, Group A: TH1 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 1, Hour 3
Cycle 3, Group A: T1 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 7 - 10
Cycle 3, Group A: TH17 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Time frame: Day 1, Hour 1
Cycle 3, Group A: TH17 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time frame: Day 1, Hour 3
Cycle 3, Group A: TH17 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time frame: Day 7 - 10
Cycle 3, Group A: T-reg cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 3, Group A: T-reg cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 3, Group A: T-reg cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 7-10
Cycle 3, Group B: TH1 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 3, Group B: TH1 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 3, Group B: TH1 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 7-10
Cycle 3, Group B: TH17 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 3, Group B: TH17 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 3, Group B: TH17 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 7-10
Cycle 3, Group B: T-reg cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 3, Group B: T-reg cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 3, Group B: T-reg cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 7-10
Cycle 4, Group A: T1 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 4, Group A: T1 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 4, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 7 - 10
Cycle 4, Group A: TH17 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 4, Group A: TH17 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 4, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 peripheral lymphocyte cells will be measured in %.
Time frame: Day 7 - 10
Cycle 4, Group A: T-reg cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 4, Group A: T-reg cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 4, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg peripheral lymphocyte cells will be measured in %.
Time frame: Day 7 - 10
Cycle 4, Group B : TH1 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 4, Group B : TH1 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 4, Group B : TH1 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 7 -10
Cycle 4, Group B : TH17 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 4, Group B : TH17 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 4, Group B : T17 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 7 -10
Cycle 4, Group B : T-reg cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 4, Group B : T-reg cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 4, Group B : T-reg cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 7 - 10
Cycle 5, Group A : TH1 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 5, Group A : TH1 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 5, Group A : TH1 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.
Time frame: Day 7 - 10
Cycle 5, Group A : TH17 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 5, Group A : TH17 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 5, Group A : TH17 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time frame: Day 7 - 10
Cycle 5, Group A : T-reg cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 5, Group A : T-reg cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 5, Group A : T-reg cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 7 - 10
Cycle 5, Group B : TH1 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 5, Group B : TH1 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 5, Group B : TH1 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 7-10
Cycle 5, Group B : TH17 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 5, Group B : TH17 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 5, Group B : TH17 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 7-10
Cycle 5, Group B : T-reg cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 0
Cycle 5, Group B : T-reg cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 0, Hour 3
Cycle 5, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 7-10
Cycle 6, Group A : TH1 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0 Hour 0
Cycle 6, Group A : TH1 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0 Hour 3
Cycle 6, Group A : TH1 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 7-10
Cycle 6, Group A : TH17 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 0 Hour 0
Cycle 6, Group A : TH17 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 0 Hour 3
Cycle 6, Group A : TH17 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time frame: Day 7-10
Cycle 6, Group A : T-reg cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 0 Hour 0
Cycle 6, Group A : T-reg cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 0 Hour 3
Cycle 6, Group A : T-reg cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time frame: Day 7-10
Cycle 6, Group B : TH1 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0 Hour 0
Cycle 6, Group B : TH1 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 0 Hour 3
Cycle 6, Group B : TH1 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time frame: Day 7-10
Cycle 6, Group B : TH17 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Time frame: Day 0 Hour 0
Cycle 6, Group B : TH17 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time frame: Day 0 Hour 3
Cycle 6, Group B : TH17 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time frame: Day 7-10
Cycle 6, Group B : T-reg cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 0 Hour 0
Cycle 6, Group B : T-reg cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 0 Hour 3
Cycle 6, Group B : T-reg cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time frame: Day 7-10
Cycle 1, Group A : T4 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group A : T4-DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group A : T8 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group A : B lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.
Cycle 1, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group A : T4 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group A : T4-DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group A : T8 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group A : T8 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group A : B lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group A : CD45RA-CCR7- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group A : CR6-CXCR3- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group A : T4-DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group A : B lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group B: T4 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group B: T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group B: T8 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group B: T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group B: B lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group B: CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group B: CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group B: CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group B: CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 1, Group B:T4 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group B:T4 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group B:T8 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group B:T8 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group B: B lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group B: CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group B: CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group B: CD45RA-CCR7- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group B: CCR6-CXCR3- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 1, Group B: T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group B: T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group B: T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group B: T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group B: B lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group B: CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group B: CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group B: CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 1, Group B: CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 2, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group A : B lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group A : T4 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group A : T8 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group A : B lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7 - 10
Cycle 2, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7 - 10
Cycle 2, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7 - 10
Cycle 2, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7 - 10
Cycle 2, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7 - 10
Cycle 2, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7 - 10
Cycle 2, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7 - 10
Cycle 2, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7 - 10
Cycle 2, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7 - 10
Cycle 2, Group B : T4 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group B : T8 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group B : B lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 2, Group B : T4 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group B : T4 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group B : T8 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group B : T8 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group B : B lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group B : CD45RA-CCR7- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group B : CCR6-CXCR3- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 2, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 2, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 2, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 2, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 2, Group B : B lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 2, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 2, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 2, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured a percentage.
Time frame: Day 7-10
Cycle 2, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group A : T4 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group A : T8 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group A : B lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group A : CC45RA+CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group A : CC45RA-CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group A : CC45RA-CCR7- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group A : T4 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group A : T4 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group A : T8 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group A : T8 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group A : B lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group A : CD45RA-CCR7- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group A : CCR6-CXCR3- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group A : T4 lymphocytes 7 -10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group A : B lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group B : B lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 3, Group B : T4 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group B : T8 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group B : B lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 3, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 3, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group A : B lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group A : T4 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group A : T8 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group A : B lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group B : T4 lymphocytes BEFORE plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group B : T4 DR+ lymphocytes BEFORE plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group B : T8 lymphocytes BEFORE plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group B : B lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 0
Cycle 4, Group B : T4 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group B : T4 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group B : T8 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group B : T8 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group B : B lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group B : CD45RA-CCR7- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group B : CCR6-CXCR3- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0 Hour 3
Cycle 4, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group B : B lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 4, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group A : T4 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group A : T8 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group A : B lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group A : T4 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group A : T4 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group A : T8 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group A : T8 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group A : B lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group A : CD45RA-CCR7- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group A : CCR6-CXCR3- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group A : B lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group B : B lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 5, Group B : T4 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group B : T8 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group B : B lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 5, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 5, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 6, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 6, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 6, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 6, Group A : B lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 6, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 6, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 6, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 6, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 0
Cycle 6, Group A : T4 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 6, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 6, Group A : T8 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 6, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 6, Group A : B lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 6, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 6, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 6, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 6, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, Hour 3
Cycle 6, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group B : T4 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H0
Cycle 6, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H0
Cycle 6, Group B : T8 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H0
Cycle 6, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H0
Cycle 6, Group B : B lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H0
Cycle 6, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H0
Cycle 6, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H0
Cycle 6, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H0
Cycle 6, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H0
Cycle 6, Group B : T4 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H3
Cycle 6, Group B : T4 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H3
Cycle 6, Group B : T8 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H3
Cycle 6, Group B : T8 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H3
Cycle 6, Group B : B lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H3
Cycle 6, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H3
Cycle 6, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H3
Cycle 6, Group B : CD45RA-CCR7- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H3
Cycle 6, Group B : CCR6-CXCR3- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 0, H3
Cycle 6, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group B : B lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10
Cycle 6, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time frame: Day 7-10