The Culture of Ovarian Cancer Organoids and Drug Screening

Inclusion Criteria: 1. Ovarian cancer patients who will accept primary cytoreductive surgery, with histopathological type: low/high grade serous carcinoma, clear cell carcinoma, endometrioid carcinoma, mucinous carcinoma. 2. ECOG score 0\~1,age 18\~70 years old 3. Expected survival over 6 months 4. The serum or urine pregnancy test must be negative within 7 days before enrollment for the women of childbearing age who should agree that contraception must be used during the trial 5. CBC：Hb≥70g/L、WBC≥3.5×109/ L 、ANC≥1.5×109/L、PLT≥80×109/L； 6. Serum ALT≤2×UL, AST≤2×ULN；Serum creatinine≤1.5×ULN； Exclusion Criteria: 1. Activity or uncontrol severe infection 2. Liver cirrhosis, Decompensated liver disease 3. History of immune deficiency, including HIV positive or suffering from congenital immunodeficiency disease 4. Chronic renal insufficiency or renal failure 5. Has combined with other malignant tumor which diagnosed within 5 years and/or needed to be treated 6. Myocardial infarction, severe arrhythmia and NYHA (New York heart association)≥2 for congestive heart failure 7. During the treatment for complications, the drugs which lead to serious liver and/or kidney function impairment need to be used, such as tuberculosis