Does Notch Regulate Glioma Proliferation ?

University Hospital, Montpellier10 enrolled

Overview

The investigators showed in 2015 (Guichet et al) that the Notch1 pathway has an anti-proliferative role on glial brain tumors. In this project, the investigators investigated which genes downstream of Notch1 pathway activation produce the anti-proliferative effect. The investigators hypothesized that the SNAI2 and TAL1 genes act downstream of Notch1

Main objectives: To examine the expression of TAL1 and SNAI2 in protein extracts and glioma sections of different grades (glioblastomas and diffuse low-grade tumors). To examine the effect of overexpression of Tal1 and Snai2 in high grade glioma cultures. To show the expression of Tal1 and SNAI2 by tumor cells by FISH technique. Show that TAL1 and SNAI2 could be regulated by endothelial cells

Study Type

OBSERVATIONAL

Enrollment

Conditions

Glioma

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion criteria: * Patient ≥18 years old * Patient affected with brain glioma classified according to WHO 2016 classification Exclusion Criteria: * Patient who reject the study protocol * Radiotheray or chemotherapy treatment

Locations (1)

Uhmontpellier

Montpellier, France

Outcomes

Primary Outcomes

Effect of Notch1 activation on Tal1

highthroughput gene analysis (DNA arrays) with cells transduced with activated form of Notch1

Time frame: 1 day

Snai2 gene and protein expression

western blot for Tal1 and Snai2 in protein extracted from low grade and high gliomas

Time frame: 1 day

Data from ClinicalTrials.gov

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