This study is a retrospective record review to determine whether a novel multiplex PCR assay can be utilized as a screening method for UTI in patients receiving Botox injection to treat overactive bladder (OAB).
The novel multiplex real-time PCR assay with mixed floral antibiotic resistance profiling offers a higher degree of sensitivity and specificity than conventional culture methods in the identification of UTI pathogens as determined by a previous prospective comparison study. This is a retrospective record review comparing PCR and culture-based assessments for UTI in patients receiving Botox injection to treat overactive bladder (OAB). Investigators aim to determine whether M-PCR/P-AST can be utilized as a screening method for UTI in these patients and whether whether antibiotic prophylaxis, peri-procedural antibiotics, and post-injection antibiotic therapy based on patients' pre-procedural M-PCR/P-AST results in improved response to Botox injection compared to standard of care. The attributes and advantages of PCR testing over the use of traditional urine cultures, and the potential identification of polymicrobial infections with complicated resistance sharing mechanisms, may significantly improve patient care.
Study Type
OBSERVATIONAL
M-PCR/P-AST is a dual assessment test that detects pathogenic DNA commonly associated with urinary tract infections and identifies their genotypic and phenotypic antibiotic resistance.
A traditional clinical UTI evaluation consisting of non-molecular standard urine culture (SUC) and sensitivity testing methods.
Comprehensive Urologic Care
Barrington, Illinois, United States
Post procedural UTI
Defined as development of UTI confirmed by urine culture/PCR in the setting of UTI symptoms requiring antibiotic therapy
Time frame: Up to 3 weeks post injection
Recurrent UTI
Defined as the development of multiple UTI confirmed by urine culture/PCR in the setting of UTI symptoms requiring antibiotic therapy
Time frame: Up to 12 months post-injection
Resistant UTI
Defined as UTI that manifests as a persistence or worsening of symptoms during antibiotic course
Time frame: Up to 12 months post-injection
Antibiotic Usage
Defined as total number of post-procedural antibiotics ordered/taken within 3 weeks post BOTOX injection
Time frame: Up to 3 weeks post-injection
Visit Reschedule/Cancellation Rate
Defined as any injection visit cancelled/postponed/rescheduled due to results of preprocedural assessments (abnormal UA, lower urinary tract symptoms etc.)
Time frame: Up to 1 year after pre-procedural assessment
UTI-related ED Visit Rate
Defined as any visit to the ED relating to symptoms of UTI within 3 weeks post-Botox injection
Time frame: Up to 3 weeks post-injection
UTI-hospital Admission Rate
Defined as any admission to the hospital (from home/ED or clinic transfer) relating to symptoms of UTI within 3 weeks post-Botox injection
Time frame: Up to 3 weeks post-injection
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.