Enterobacteriaceae, more specifically Escherichia coli and Klebsiella pneumoniae, are the bacteria most often responsible for neonatal infections in low-income countries. Infections caused by multidrug-resistant Enterobacteriaceae: Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E), are more often associated with an unfavorable outcome of the infection. Enterobacteriaceae colonize the digestive tract which is the first step in developing a potential infection. Very few studies have been carried out at the community level. Colonization of the mother with ESBL-E is generally considered to be a major route of acquisition. The carrying of ESBL-E by other family members and other potential sources of transmission (food, objects and surfaces in contact with the newborn) have never been documented. In addition, with a view to offering an intervention adapted to the local context, the local cultural determinants which govern the interactions of the newborn with his environment are important to understand.
This is a single-center prospective cohort of all members living in the same household in which a newborn baby has just been born. The study consists to: * recruit a cohort of all members of 60 households in which a newborn has just been born * carry out semi-structured interviews and direct participant observation. This study is divided in two parts : * the epidemiological part : the documentation of colonization by E-ESBL by sampling the stools of the newborn, of all members of the household, and sampling of food given to the child, from surfaces in contact with it. * the anthropological part : Semi-structured interviews with key people in the structuring of the social and family environment of the newborn and participant direct observation of households.
Study Type
OBSERVATIONAL
Enrollment
600
Institut Pasteur de Madagascar
Antananarivo, Madagascar
RECRUITINGNumber of acquisition events and the number of non-acquisition of E-ESBL in newborns for the epidemiological part
Identification of bacterial strains from stool and sequencing of EBSL-E strains
Time frame: 2 years
Number of semi-structured interviews completed for the anthropological part
1 or 4 hours semi-structured interviews of participants at home done by investigators
Time frame: 2 years
Number of participant direct observation completed for the anthropological part
observations of the participants at home done by investigators (2 to 4 hours in 3 to 4 sessions over 28 days)
Time frame: 2 years
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