Vascular dysfunction is an important mechanism involved in organ failure, in the setting of sepsis condition, with different types of circulating endothelial cells.Transcriptom analysis via RNAseq in different types of circulating endothelial cells, comapring critically ill patients with or without sepsis will allow determining differential gene expression for signal pathways in endothelial alteration and restoration associated with sepsis.
Study Type
OBSERVATIONAL
Enrollment
1,000
Observational study. Groups are defined based on baseline patients' characteristics : presence or absence of sepsis.
AP-HP Hôpital Saint Louis
Paris, France
Gene expression based on transcriptom analysis of circulating endothelial cells
Gene expression based on transcriptom analysis of circulating endothelial cells (RNAseq)
Time frame: Day 1
Counts of circulating endothelial cells
Counts of circulating endothelial cells, including progenitor cells
Time frame: Day 1 and Day 3
Sequential Organ Failure Assessment (SOFA) score
Sequential Organ Failure Assessment (SOFA) score, ranging from 0 (best: absence of organ failure) to 24 (worse: multiple organ failure)
Time frame: Day 1, Day 3, Day 7
Sequential Organ Failure Assessment (SOFA) hemodynamic sub-score
Sequential Organ Failure Assessment (SOFA) hemodynamic sub-score, ranging from 0 (best: absence of hemodynamic failure) to 4 (worse: hemodynamic failure)
Time frame: Day 1, Day 3, Day 7
Sequential Organ Failure Assessment (SOFA) respiratory sub-score
Sequential Organ Failure Assessment (SOFA) respiratory sub-score, ranging from 0 (best: absence of respiratory failure) to 4 (worse: respiratory failure)
Time frame: Day 1, Day 3, Day 7
Sequential Organ Failure Assessment (SOFA) neurological sub-score
Sequential Organ Failure Assessment (SOFA) neurological sub-score, ranging from 0 (best: absence of neurological failure) to 4 (worse: neurological failure)
Time frame: Day 1, Day 3, Day 7
Sequential Organ Failure Assessment (SOFA) hematological sub-score
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Sequential Organ Failure Assessment (SOFA) hematological sub-score, ranging from 0 (best: absence of hematological failure) to 4 (worse: hematological failure)
Time frame: Day 1, Day 3, Day 7
Intensive care unit (ICU) mortality
Death during ICU stay
Time frame: ICU length of stay
D28 mortality
Death within 28 days of intensive care unit
Time frame: Day 28
Hospital mortality
Death within 90 days of intensive care unit
Time frame: Day 90
Duration of ventilation
Number of days alive without ventilation up to Day 28
Time frame: Day 28
Duration of renal replacement therapy (RRT)
Number of days alive without RRT up to Day 28
Time frame: Day 28
Duration of vasopressors therapy
Number of days alive without vasopressors up to Day 28
Time frame: Day 28
Dose of vasopressors
Dose of vasopressors, in patients who received vasopressors
Time frame: ICU length of stay, assessed up to 90 days
Duration of intensive care unit (ICU) stay
Number of days alive outside the ICU up to D28
Time frame: Day 28
Circulating endothelial cells cytokines profile
Dosage of cytokines from circulating endothelial cells
Time frame: Day 1 and 3
Circulating endothelial cells chemokines profile
Dosage of chemokines from circulating endothelial cells
Time frame: Day 1 and 3
Expression of membrane proteins of endothelial cells
Expression of membrane proteins of endothelial cells by flow cytometry
Time frame: Day 1 and 3
Infection site
Infection site for patients with sepsis
Time frame: Day 1
Infection identification
Identified germs for patients with sepsis
Time frame: Day 1
Number of participants with different types of antibiotic treatment
Antibiotic treatments for patients with sepsis during intensive care unit (ICU) stay
Time frame: Length of ICU stay, assessed up to 90 days