The Effect of Lifestyle-induced Hepatic Steatosis on Glucagon-stimulated Amino Acid Turnover

Inclusion Criteria: * Normal fasting plasma glucose and glycated haemoglobin (HbA1c) \<42 mmol/mol * Body mass index (BMI) between 18.5 and 25 kg/m2 * Haemoglobin \>8.3 mmol/l * Habitual diet in accordance with the Nordic Nutrition Recommendations * Age between 20 and 65 years * Oral and written informed consent Exclusion Criteria: * Diabetes * First-degree relatives with diabetes * Fasting plasma triacylglycerol indicating dyslipidemia (≥2 mmol/l) * Nephropathy (estimated glomerular filtration rate (eGFR) \<60 ml/min and/or microalbuminuria with an albumin to creatinine ratio of 30-300 μg/mg) * Known liver disease and/or alanine aminotransferase (ALT) and/or aspartate aminotransferase (AST) \>2 × normal values * Signs of liver fibrosis and/or steatosis evaluated by FibroScan (CAP value \>2380 dB/m and/or kPa \>65.0) and/or FIB-4 score (\>1.45) * \>5% steatosis evaluated by MRI carried out before experimental Day A (see Methods) * Use of medication * Use of dietary protein supplementation or any other dietary supplements that cannot be paused during participation * Excessive training habits, defined as \>2 weekly strength and/or aerobic training sessions * Pregnancy and/or breastfeeding * Implanted metal objects incompatible with magnetic resonance imaging (MRI) * Any condition that the investigator feels would interfere with trial completion