The goal of this clinical trial is to test whether our dietary intervention can prevent or lessen the negative health effects of night shift work in healthy participants. Participants will: * complete 2 inpatient stays * be provided with identical meals * have frequent blood draws * provide urine, saliva, stool and rectal swab samples
Shift work increases the risk for diabetes possibly due to the adverse metabolic effects of circadian misalignment. As shift work is not foreseen to disappear, the development of individually-targeted therapies for metabolic health in these vulnerable shift workers is urgently needed. This research will determine whether our dietary intervention can mitigate the adverse metabolic effects of circadian misalignment, which may help in the design of evidence-based dietary interventions to improve the metabolic health in shift workers.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
24
Research participants will be assigned to two dietary conditions.
Brigham and Women's Hospital
Boston, Massachusetts, United States
RECRUITINGChange in glucose tolerance from Baseline to Test Day
Change in incremental glucose area under the curve (AUC) from a mixed meal test.
Time frame: Baseline (Day 2) and Test Day (Day 4)
Changes in microbial community structure/composition/function of oral and gut microbiome from Baseline to Test Day
Shotgun metagenomics sequencing or 16S rRNA sequencing of saliva, stool, and rectal swab samples will provide in-depth interrogation of the microbial community structure, composition, and function.
Time frame: Baseline (Day 2) and Test Day (Day 4)
Change in insulin sensitivity (Oral Minimal Model method) from Baseline to Test Day
Oral Minimal Model method estimates insulin sensitivity based on blood glucose and insulin levels from a mixed meal test.
Time frame: Baseline (Day 2) and Test Day (Day 4)
Change in levels of serum markers of gut microbiota from Baseline to Test Day
Levels of markers of gut microbiota (e.g. short chain fatty acids) will be measured in serum samples taken 3-hourly over 24 hr.
Time frame: Baseline (Day 2) and Test Day (Day 4)
Change in levels of serum markers of intestinal barrier integrity from Baseline to Test Day
Levels of markers of intestinal barrier integrity (e.g. zonulin, LPS, LBP) will be measured in serum samples taken 3-hourly over 24 hr.
Time frame: Baseline (Day 2) and Test Day (Day 4)
Change in level of serum markers of inflammatory state from Baseline to Test Day
Levels of markers of inflammatory state (e.g., cytokines) will be measured in serum samples taken 3-hourly over 24 hr.
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Time frame: Baseline (Day 2) and Test Day (Day 4)