This study will compare the effects of a whey protein supplement or a placebo consumed before the evening meal on health outcomes in night shift workers.
Participants are assigned in random order to two conditions, for 12 days each. The interventions are 1) a 30g whey protein preload consumed 1-1.5 hr prior to their main evening meal every day for 12 days. No other advice will be given. 2) an identical mixed-nutrient drink matched for caloric content, taste and palatability consumed 1-1.5 hr prior to their main evening meal every day for 12 days(placebo). Conditions are separated by a 2-week washout period, during which participants will be encouraged to maintain their habitual diet and physical activity levels. Metabolic testing will be performed at baseline, and at the end of both conditions.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
DOUBLE
Enrollment
30
Protein drink containing 30g whey protein isolate powder (dissolved in water) consumed 1-1.5 hr prior to their main evening meal every day for 12 days, No other advice will be given.
Energy-matched mixed-nutrient placebo drink consumed 1-1.5 hr prior to their main evening meal every day for 12 days. No other advice will be given.
University of Adelaide
Adelaide, South Australia, Australia
RECRUITINGGlycaemic response
Change in glucose (AUC) following a standard breakfast
Time frame: 12 days (3 hours meal test)
24-hour glucose profiles
Change in 24-hour glucose profiles assessed by continuous glucose monitoring
Time frame: 12 days
Glucose variability
Change in 24-hour glucose variability (as SD, standard deviation) assessed by continuous glucose monitoring
Time frame: 12 days
Glucose variability
Change in 24-hour glucose variability (as CV, coefficients of variation) assessed by continuous glucose monitoring
Time frame: 12 days
Glucose variability
Change in 24-hour glucose variability (as MAGE, mean amplitude of gylcaemic excursions) assessed by continuous glucose monitoring
Time frame: 12 days
GLP-1
Change in GLP-1 (AUC) following a standard breakfast
Time frame: 12 days (3 hours meal test)
GIP
Change in GIP (AUC) following a standard breakfast
Time frame: 12 days (3 hours meal test)
glucagon
Change in glucagon (AUC) following a standard breakfast
Time frame: 12 days (3 hours meal test)
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Insulin
Change in fasting and postprandial insulin following a standard breakfast
Time frame: 12 days
Ghrelin
Change in ghrelin (AUC) following a standard breakfast
Time frame: 12 days (3 hours meal test)
PYY
Change in YY (AUC) following a standard breakfast
Time frame: 12 days
Adiponectin
Change in fasting adiponectin
Time frame: 12 days
C-Reactive Protein (CRP)
Change in fasting CRP
Time frame: 12 days
Blood lipids
Changes in blood lipid profile (total cholesterol, HDL-, LDL-cholesterol and triglycerides)
Time frame: 12 days
Blood pressure
Changes in systolic blood pressure and diastolic blood pressure
Time frame: 12 days
Resting metabolic rate
Changes in resting metabolic rate
Time frame: 12 days
respiratory quotient
Changes in respiratory quotient
Time frame: 12 days